·28·医药前沿2024年3月第14卷第9期论著某校大学生梭状芽孢杆菌携带情况调查刘梦昭1，苏诗洁1，黄贞维1，苏嘉雯1，容欣1，王岐本2，谢群1（通信作者）（1湘南学院公共卫生学院湖南郴州423000）（2湘南学院基础医学院湖南郴州423000）【摘要】目的：通过对湘南学院大学生粪便中梭状芽孢杆菌的分离培养及鉴定，了解大学生梭状芽孢杆菌的携带情况。方法：2022年3—6月收集湘南学院大学生粪便样本180份，厌氧培养法分离艰难梭菌、产气荚膜梭菌和丁酸梭菌，血平板纯化菌株，通过聚合酶链式反应（PCR）法鉴定梭状芽孢杆菌，对可疑艰难梭菌进行毒素基因A/B检测。采用16SrDNA技术对分离的菌株进行鉴定。结果：艰难梭菌A/B毒素基因检出13份阳性，均为+/+，30份可疑菌株16SrDNA确认：11份产气荚膜梭菌、2份丁酸梭菌、2份艰难梭菌。结论：湘南学院大学生人群中，产气荚膜梭菌携带率较高，艰难梭菌感染率较高，需加强食品安全健康教育，避免食源性疾病发生。【关键词】大学生；梭状芽孢杆菌；艰难梭菌；产气荚膜梭菌；丁酸梭菌【中图分类号】R378.8【文献标识码】A【文章编号】2095-1752（2024）09-0028-03InvestigationonClostridiumcarryingofcollegestudentsinAUniversityLIUMengzhao1,SUShijie1,HUANGZhenwei1,SUJiaWen1,RONGXin1,WANGQiben2,XIEQun1(Correspondingauthor)1SchoolofPublicHealth,XiangnanUniversity,Chenzhou,Hunan423000,China2SchoolofbasicMedicalSciences,XiangnanUniversity,Chenzhou,Hunan423000,China【Abstract】ObjectiveTounderstandthecarriagesituationofClostridiumdifficileamongcollegestudentsthroughisolation,culture,andidentificationofClostridiumdifficileinfecalsamplesfromXiangnanUniversity.MethodsFromMarchtoJune2022,180fecalsampleswerecollectedfromXiangnanUniversitystudents.AnaerobicculturemethodwasusedtoisolateClostridiumdifficile,Clostridiumperfringens,andClostridiumbutyricum.Thestrainswerepurifiedonbloodagarplates,identifiedusingpolymerasechainreaction(PCR),andsuspectedClostridiumdifficileweretestedfortoxingenesA/b.16SrDNAtechnologywasusedforstrainidentification.Results13sampleswerepositivefortoxingenesA/bofClostridiumdifficile,allofwhichwere+/+.16SrDNAconfirmed30suspectedstrains:11wereClostridiumperfringens,2wereClostridiumbutyricum,and2wereClostridiu...
