《食品工业》2023年第44卷第2期102工艺技术滇藏杜英总酚提取方法的筛选田素梅*，李兆云，马艳粉德宏师范高等专科学校（芒市678400）摘要采用正交试验设计L9(34),研究不同因素对滇藏杜英(Elaeocarpusbraceanus)总酚提取率的影响。比较和综合评价了乙醇浸渍法、回流提取法和超声波提取法三种提取方法对滇藏杜英总酚提取率的差异。直观分析结果表明:运用乙醇浸渍法提取时,采用料液比1∶30(g/mL)、乙醇80%,提取次数3次的组合方案提取率最高;回流提取法的最佳组合方案为料液比1∶50(g/mL)、提取温度100℃,提取时间2h;利用超声波提取法提取时,因素最佳组合为提取温度70℃、料液比1∶20(g/mL)、提取次数1次。方差分析结果表明,提取次数对乙醇浸渍法和超声波提取法的提取率有显著影响(P＜0.05),料液比和提取温度对超声波提取法的提取率有显著影响(P＜0.05)。回流提取法的总酚提取率显著高于乙醇浸渍法和超声波提取法(P＜0.05)的提取率。综合评价结果显示回流提取法的总酚提取率效果最佳。关键词滇藏杜英;总酚;正交试验;隶属函数EvaluationofExtractionMethodsofTotalPhenolsofElaeocarpusbraceanusTIANSumei*,LIZhaoyun,MAYanfenDehongJuniorNormalCollege(Mangshi678400)AbstractOrthogonalexperimentL9(34)wasusedtostudytheeffectsofdifferentfactorsontheextractionrateoftotalphenolsfromElaeocarpusbraceanus.ThedifferencesofextractionratesoftotalphenolsfromElaeocarpusbraceanuswerecomparedandcomprehensivelyevaluatedbythreeextractionmethodsofethanolimmersion,refluxextractionandultrasonicextraction.Theintuitiveanalysisresultsshowedthatwhenusingethanolimpregnationextractionmethod,thecombinedschemeofsolidliquidratioof1∶30(g/mL),ethanol80%,extractiontimesof3timeswasthehighestextractionrate.Theoptimalcombinationschemeofrefluxextractionmethodwasasfollows:solid-liquidratioof1∶50(g/mL),extractiontemperatureof100℃,andextractiontimeof2h.Whenusingultrasonicextractionmethod,thebestcombinationoffactorswasextractiontemperatureof70℃,solid-liquidratioof1∶20(g/mL),andextractiontimes1time.TheresultsofANOVAshowedthattheextractiontimeshadasignificanteffectontheextractionrateofethanolimmersionandultrasonicextraction(P＜0.05),andtheratioofsolidtoliquidandextractiontemperaturehadasignif...
