Biomaterials27(2006)3238–3248Performanceofcollagenspongeasa3-Dscaffoldfortooth-tissueengineeringYoshinoriSumitaa,b,MasakiJ.Hondaa,�,TakayukiOharac,ShuheiTsuchiyaa,b,HiroshiSagarad,HideakiKagamie,MinoruUedaa,baToothRegeneration,DivisionofStemCellEngineering,TheInstituteofMedicalScience,TheUniversityofTokyo,4-6-1Shirokanedai,Minato-ku,Tokyo108-8639,JapanbDepartmentofOralandMaxillofacialSurgery,NagoyaUniversityGraduateSchoolofMedicine,65Tsuruma-cho,Showa-ku,Nagoya466-8550,JapancResearch&DevelopmentCenter,HitachiMedicalCorporation,2-1ShintoyofutaKashiwa277-0804,JapandLaboratoryofFineMorphology,TheInstituteofMedicalScience,TheUniversityofTokyo,4-6-1Shirokanedai,Minato-ku,Tokyo108-8639,JapaneDepartmentofTissueEngineering,NagoyaUniversitySchoolofMedicine,65Tsuruma-cho,Showa-ku,Nagoya466-8550,JapanReceived29November2005;accepted24January2006AbstractToothstructurecanberegeneratedbyseedingdissociatedtoothcellsontopolyglycolicacidfibermesh,althoughthesuccessrateoftoothproductionislow.Thepresentstudywasdesignedtocomparetheperformanceofcollagenspongewithpolyglycolicacidfibermeshasa3-Dscaffoldfortooth-tissueengineering.Porcinethirdmolarteethattheearlystageofcrownformationwereenzymaticallydissociatedintosinglecells,andtheheterogeneouscellswereseededontocollagenspongeorthepolyglycolicacidfibermeshscaffolds.ScaffoldswerethenculturedtoevaluatecelladhesionandALPactivityinvitro.Aninvivoanalysiswasperformedbyimplantingtheconstructsintotheomentumofimmunocompromisedratsandevaluatingtoothproductionupto25weeks.After24h,therewereasignificantlyhighernumberofcellsattachedtothecollagenspongescaffoldthanthepolyglycolicacidfibermeshscaffold.Similarly,theALPactivitywassignificantlyhigherforthecollagenspongescaffoldwasthanthepolyglycolicacidfibermeshscaffoldafter7daysofculture.Theareaofcalcifiedtissueformedinthecollagenspongescaffoldwasalsolargerthaninthepolyglycolicacidfibermeshscaffold.Theresultsfrominvivoexperimentsshowconclusivelythatacollagenspongescaffoldallowstoothproductionwithahigherd...
