117AbstractInthischapter,wereviewthediscoveryofMAP30,anovelmulti-functionalantiviralandanti-tumorplantprotein,andelucidationofitsmedici-nalproperties.MAP30,MomordicaAntiviralProtein30kD,isisolatedfromthemedicinalplantMomordicacharantia.Biochemicalandbiophysicalstudieshaveyieldedimportantinsightsintothestructure,functionandmechanismofMAP30.Columnchromatographybyionicexchange,affinitybindingandsizefractionationwereappliedfortheisolationandpurificationofMAP30.Proteinandnucleo-tidesequencingwereusedtoelucidatethestructureofthisproteinanditsgene.ThegeneencodingMAP30wasclonedandexpressedbyrecombinanttechnology.Structureactivitymappingwasperformedusing3D-structuredetermination,bioinformaticsandmolecularmodeling,togetherwithbioactivityconfirmation.Resultsfromthesestudiesdemonstratehowcutting-edgemodernapproachescanbeappliedtodiscovermedicinalactivitiesfromnaturalproductsandtodefinetheirmolecularstructure,biologicalfunction,andmechanismsofaction.Chapter8TheDiscoveryofMAP30andElucidationofitsMedicinalActivitiesSylviaLee-Huang,PhilipLinHuangandPaulLeeHuangE.F.FangandT.B.Ng(eds.),AntitumorPotentialandOtherEmergingMedicinalPropertiesofNaturalCompounds,DOI:10.1007/978-94-007-6214-5_8,©SpringerScience+BusinessMediaDordrecht2013S.Lee-Huang(*)DepartmentofBiochemistryandMolecularPharmacology,NewYorkUniversitySchoolofMedicine,NewYork,NY10016,USAe-mail:sylvia.lee-huang@med.nyu.eduP.LinHuangAmericanBioScience,Boston,MA,USAP.LeeHuangDepartmentofMedicine,HarvardMedicalSchoolandMassachusettsGeneralHospital,Boston,MA02114,USAThisChapterisdedicatedtothememoryofProfessorSeveroOchoa,ouradoredmentor,andtoDr.AnFuLee,ourbelovedmotherandgrandmother,fortheirinspirationandencouragement.118S.Lee-Huangetal.8.1IntroductionOver75%oftheworldpopulationreliesmainlyonmedicinalplantsforhealthcare[1].TheglobalmarketfortraditionalmedicineswasestimatedatUS$83bil-lionannuallyin2008[1].IntheUnitedStates,botanicalextractsanddietarysupplementsareregardedaspartofcomplementaryandalternativemedicine(CAM)becausethe...
