AgriculturalBiotechnology2014，3(4):12－13PlantScienceOptimizationofUltrasonicExtractionProcessofTotalFlavonoidsfromMulberryLeavesbyOrthogonalExperi-mentXianlingWANG*，YaningWANG，XiuxiuYANG，MinXUSchoolofPharmacy，HenanUniversityofTraditionalChineseMedicine，Zhengzhou450046，ChinaAbstractInthisstudy，byusingmulberryleavesasrawmaterials，ultrasonicextractionprocessoftotalflavonoidsfrommulberryleaveswasoptimizedbyorthogo-nalexperiment．Ｒesultsshowedthatthemaximumyieldoftotalflavonoids(6．1%)wasobtainedfrommulberryleaveswithadditionof45timesthevolumeofwaterbyultrasonicextractionat55℃forthreetimes，55mineach．Accordingtotheverificationtest，theoptimizedtechnicalprocesswasreasonableandfeasiblewithhighstability．KeywordsOrthogonalexperiment;Mulberryleaves;Totalflavonoids;UltrasonicextractionMulberryleaves，referredtoasdriedleavesofMoraceaeplantMorusalbaL．Generally，mulberryleavesareharvestedaf-MaterialsandMethodsterthefirstfrostanddriedwithremovalofimpurities，whichareExperimentalmaterialsＲutinstandard:purchasedfromNationalInstituteforthesweetandbitterinflavor，coldinproperty，andenterthelungandControlofPharmaceuticalandBiologicalProducts，batchnumber:livermeridian［1］．Mulberryleavespossessfunctionsofdispelling100080-200707;leaves:windandclearingawayheat，clearinglungandmoisturizingdry-mulberrypurchasedfromZhangZhongjingPharmacy，whichwereidentifiedasdriedleavesofMo-ness，soothingliverandeyesight，coolingbloodandimprovingrusalbaL．;ethanol，sodiumnitrite，aluminumnitrate，sodiumstopbleeding，whichcananemopyreticcold，beusedtotreathydroxideandotherreagentswereallofanalyticalgrade;congestion，drynessheatimpairingdistilledheadachewithconjunctivallung，coughwithlittlephlegm，liver-yanghyperactivity，dizzying，waterwaspreparedinourlaboratory．blood-heat，hematemesisleaves，Instrumentsandotherdiseases．MulberryUltrasoniccleaningmachine，ZhengzhouShengYuanInstru-branches，rootbarksandfruitallhavecertainmedicinalvalue．mentCo．，Ltd．;SHZ-D(III)vacuumpump，GongyiYuhuaIn...
