Biodegradablepolymerwithcollagenmicrospongeservesasanewbioengineeredcardiovascularprosthesis生物降解高分子材料胶原海绵作为一种新的生物工程心血管修复材料复合ShigemitsuIwai,MDa；YoshikiSawa,MDa；HajimeIchikawa,MDa；SatoshiTaketani,MDa；EiichiroUchimura,PhDb；GuopingChen,PhDb；MasayukiHara,PhDc；JunMiyake,PhDb；HikaruMatsuda,MDaMD医学博士；PhD理学博士Objective:Biodegradablematerialswithautologouscellseedinghaveattractedmuchinterestaspotentialcardiovasculargrafts.However,pretreatmentofthesematerialsrequiresacomplicatedandinvasiveprocedurethatcarriestheriskofinfection.Toavoidtheseproblems,wesoughttodevelopabiodegradablegraftmaterialcontainingcollagenmicrospongethatwouldpermittheregenerationofautologousvesseltissue.Theabilityofthismaterialtoaccelerateinsitucellularizationwithautologousendothelialandsmoothmusclecellswastestedwithandwithoutprecellularization.目标：作为潜在的一种心血管桥，含自体细胞接种的生物降解材料吸引了许多关注。然而，这些材料的预处理需要一个复杂和有扩散危害的程序并具有被感染的危险。为了避免这些问题，我们试图开发一种包含胶原蛋白海绵的具有生物降解作用的嫁接材料会允许自体血管组织再生。这种材料在原有位置加速自体内皮平整的肌肉组织细胞的细胞化的能力被用来测试有无先细胞化。Methods:Poly(lactic-co-glycolicacid)asabiodegradablescaffoldwascompoundedwithcollagenmicrospongetoformavascularpatchmaterial.Thesepoly(lactic-co-glycolicacid)–collagenpatcheswith(n=10)orwithout(n=10)autologousvesselcellularizationwereusedtopatchthecaninepulmonaryarterytrunk.Histologicandbiochemicalassessmentswereperformed2and6monthsaftertheimplantation.方法：多聚丙交酯-乙交酯作为一种生物降解支架被与胶原蛋白海绵混合从而构成一个血管补片材料。这些含(n=10)或不含(n=10)的自体血管细胞化的多聚丙交酯-乙交酯---胶原蛋白补片曾被用于补充犬类的肝动脉主干。组织学与生物化学评估结果在培植后的2-6个月后表现出来。Results:Therewasnothrombusformationineithergroup,andthepoly(lactic-co-glycolicacid)scaffoldwasalmostcompletelyabsorbedinbothgroups.Histologicresultsshowedtheformationofanendothelialcellmonolayer,apa...
