TransfectionSelectionToolTransfectionofDNA,siRNA,oligonucleotides,andRNAintomammaliancellsisacriticalstepinmanyresearcheffortsofthebiomedicalscientificcommunityincludinggenesilencingusingRNAi,transientgeneexpressionstudies,protein/antibodyproduction,andgenerationofstablecelllines.However,successfultransfectionisnotrivialmatter.Tosimplifythetransfectionoptimizationprocess,wehavecreatedatransfectionselectiontoolthatprovidesrecommendedtransfectionreagentwithoptimizedprotocolfor120celllines.Usethefollowingtablestolocateyourcelltype(s)ofinterest,application,recommendedtransfectionreagent,andareferenceprotocol.SelectaCellType:0-9|A-F|G-H|I-M|N-R|S-W|ShowAllCellTypeSourceApplicationRecommendedTransfectionReagentRecommendedTransfectionSKUProtocol/Reference0-910T1/2MouseembryonicfibroblastPlasmidDNALipofectamine™andPLUS™Reagent18324-01218324-02011514-015293-FHumankidneyProteinProduction293fectin™12347-019293THumanembryonickidneysiRNALipofectamine™RNAiMAX13778-07513778-1503T3L1MousefibroblastPlasmidDNALipofectamine™LTXandPLUS™Reagent15338-10015338-50011514-0153T3L1MousefibroblastsiRNALipofectamine™200011668-01911668-02711668-500786-OHumanrenalcellsiRNALipofectamine™13778-07513778-150adenocarcinomacellsRNAiMAXA-FA549HumanlungcarcinomaPlasmidDNALipofectamine™200011668-01911668-02711668-500A549HumanlungcarcinomasiRNALipofectamine™RNAiMAX13778-07513778-150ACHNHumanrenalcancerPlasmidDNALipofectamine™LTXandPLUS™Reagent15338-10015338-50011514-015ARPE-19HumanretinaPlasmidDNALipofectamine™LTX15338-10015338-500B16-F10MousemelanomaPlasmidDNALipofectamine™18324-01218324-020BAECBovineaorticendothelialcellssiRNALipofectamine™200011668-01911668-02711668-500BGOV-1HumanembryonicstemcellsPlasmidDNALipofectamine™LTXandPLUS™Reagent15338-10015338-50011514-015BHK-21HamsterkidneyPlasmidDNALipofectamine™200011668-01911668-02711668-500BreastAdiposeFibroblastsHumanbreastadiposesiRNALipofectamine™RNAiMAX13778-07513778-150C2C12MousemusclemyoblastPlasmidDNALipofectamine™200011668...
