511AsianPacificJournalofTropicalMedicine(2012)511-513Documentheadingdoi:MolecularcloningofvirB12geneofBrucellamelitensis16MstraininpET28avectorShivaMirkalantari1,NourAmirmozafari1*,BahramKazemi2,GholamrezaIrajian11TehranUniversityofMedicalSciences,SchoolofMedicine,MicrobiologyDepartment,Tehran,Iran2ShahidBeheshtiUniversityofMedicalSciences,DepartmentofBiotechnology,Tehran,IranContentslistsavailableatScienceDirectAsianPacificJournalofTropicalMedicinejournalhomepage:www.elsevier.com/locate/apjtmARTICLEINFOABSTRACTArticlehistory:RReceived7November2011Receivedinrevisedform27January2012Accepted15March2012Availableonline20July2012Keywords:BrucellaCloningvirB12pET28apJE1.2*Correspondingauthor:NourAmirmozafari,TehranUniversityofMedicalSciences,SchoolofMedicine,MicrobiologyDepartment,Tehran,Iran.Tel:+982188058649Fax:+982188058649E-mail:Amirmozafari@yahoo.com1.IntroductionBrucellosisisawidespreadzoonoticinfectiousdiseasethatisacquiredbyhumansprimarilythroughcontactwithinfectedabortion-relatedanimaltissuesandcontaminateddairyproducts.Itcanhavediverseclinicalmanifestationswithsymptomsthatoverlapwithotherdiseases[1-3].Owingtothefactthatbacteriologicalmethodsarenotsensitiveenoughforbrocellusisdetection[4,5],serologicaltestsareoftenthepreferredmethodfordiagnosisofBrucellosisinbothhumansandanimals[6].Sincetheavailableserologicaltestsdetectcirculatingantibodiestobacteriallipopolysaccharide,thesetestssufferfromextensivecrossreactionwithotherGram-negativebacteria;therefore,interestinfindingalternativeandmorespecificbacterialantigentodetectbrucellosisisontherise[7-10].SeveralproteinsfromBrucellamelitensis(B.melitensis)werefoundtoinduceanantibodyresponseininfectedanimalsandhumans[11-13].ItwasrecentlyreportedthatVirB12protein,asurface-localizedproteinofBrucellaspp.elicitantibodyresponsesinbothexperimentallyandnaturallyinfectedanimals[2,14].TheaimofthisstudywascloningofvirB12geneinpET28aexpressionvectorforproductionofrecombinantproteintobeusedasantigeniccomponentforfutureserologicaltes...
