JournalofVisualizedExperimentswww.jove.comCopyright©2010JournalofVisualizedExperimentsandAgilentTechnologies,Inc.Page1of1MaterialsListfor:DNA-basedFishSpeciesIdentificationProtocolRachelFormosa1,HariniRavi1,ScottHappe1,DanielleHuffman1,NataliaNovoradovskaya1,RobertKincaid1,SteveGarrett11AgilentTechnologiesCorrespondenceto:RachelFormosaatrachel.formosa@agilent.comURL:http://www.jove.com/video/1871DOI:doi:10.3791/1871MaterialsNameCompanyCatalogNumberCommentsAgilent2100Bioanalyzer(withchipprimingstationandIKAvortexmixer)AgilentTechnologiesG2939AAAgilent2100Bioanalyzerlaptop&ExpertSoftwareAgilentTechnologiesG2953CAG2950CA-desktopPC&ExpertSoftwareAgilentBioanalyzerDNA1000ReagentKit(part#5067-1504)AgilentTechnologies5067-1504ThermalCyclerVariousVariousPCRStripTubesAgilentTechnologies401428PCRStripCapsAgilentTechnologies40142596-WellWorkingRackAgilentTechnologies410094FishSpeciesIdentificationSystemDNAIsolationKitAgilentTechnologiesContactFishSpeciesIdentificationSystemPCR-RFLPReagentKitAgilentTechnologiesContactRFLPMatcherSoftwareAgilentTechnologiesContact100%ethanol,200proof(USPgradeorequivalent)VariousVariousSterile,nuclease-freewaterVariousVariousPipettorsVariousVariousIncubatororwaterbathsetto65°CVariousVariousSterileglassbottleorpolypropylenetube(e.g.14-mlBDFalconpolypropylene)VariousVariousround-bottomtubesor50-mlBDFalconpolypropyleneconicaltubes)VariousVariousVortexmixerVariousVariousMicrocentrifugeVariousVariousMicrocentrifugetubes1.5mlVariousVariousTuberack1.5mlVariousVariousPipetteTipsVariousVarious
