(c)2007:MilliporeCorporation.Allrightsreserved.Nopartoftheseworksmaybereproducedinanyformwithoutpermissioninwriting.Rev.B/12-20-07/JMMagnaChIP™A(Catalog#17-610)EZ-MagnaChIP™A(Catalog#17-408)One-DayChromatinImmunoprecipitationKitsInstructionManualEachkitcontainssufficientreagentsfor22chromatinimmunoprecipitation(ChIP)assays.ContentsPageI.MAGNACHIP™PRODUCTKITCONFIGURATIONS2II.KITDESCRIPTION2III.MAGNACHIP™KITCOMPONENTS3IV.CHROMATINIMMUNOPRECIPITATIONBACKGROUND5V.CHROMATINIPASSAYOVERVIEW6VI.COMPARISONOFSTANDARDANDRAPIDMAGNACHIP™PROTOCOLS7VII.MAGNACHIP™PRODUCTPERFORMANCE8VIII.DETAILEDCHROMATINIMMUNOPRECIPITATIONPROTOCOL9A.InvivoCrosslinkingandLysis9B.SonicationtoShearDNA10C.Immunoprecipitation(IP)ofCrosslinkedProtein/DNA11D.ElutionandReverseCrosslinksofProtein/DNAComplexes12E.DNAPurificationusingSpinColumns12F.PCRofControls13IX.APPENDIXA–OptimizationofDNASonication16X.APPENDIXB–FormaldehydePreparation18XI.CHROMATINIPOPTIMIZATIONANDTROUBLESHOOTING19Salesorders:8002333991CallcollectfromoutsidetheU.S.4349754300Techsupport:8005487853Faxorders:8668313991www.millipore.comFORRESEARCHUSEONLY.DONOTUSEINDIAGNOSTICPROCEDURES.MagnaChIP™A-Catalog#17-610Page2of23EZ-MagnaChIP™A-Catalog#17-408TechSupport1-800-548-7853www.millipore.comMagnaChIP™andEZ-MagnaChIP™Kitsareavailableinseveralformatssoyoucaneasilyselecttheoptimalkitforyourresearchneeds.I.MagnaChIPTM&EZ-MagnaChIPTMKitConfigurationsMagnaChIPTMA(Cat.#17-610)MagnaChIPTMG(Cat.#17-611)EZ-MagnaChIPTMA(Cat.#17-408)EZ-MagnaChIPTMG(Cat.#17-409)MAGNA0001(Storeat4°C)MAGNA0002(Storeat4°C)MAGNA0001(Storeat4°C)MAGNA0002(Storeat4°C)MAGNA0003(Storeat-20°C)MAGNA0003(Storeat-20°C)MAGNA0004(Storeat-20°C)MAGNA0005(Storeat-20°C)II.KitDescriptionQuantity:Twoboxescontainingallnecessaryreagentstoperform22individualchromatinimmunoprecipitation(ChIP)reactions.Suppliedbuffersaresufficienttogeneratechromatinfromuptofive15cmplatesofculturedcells,eachplateprovidingupto10chromatinpreparations(varieswithcellandassaytype).StorageandStabili...
