BDBiosciencesFebruary16,20113DCellCultureSystemsMarshallJ.Kosovsky,Ph.D.2TopicsforDiscussion•OverviewofCellCultureSystems:2Dvs.3D•ExtracellularMatrix(ECM)Proteins–Epithelialcelldifferentiation–Endothelialcelltubeformation•Animal-FreeMatrix:BD™PuraMatrix™PeptideHydrogel–Primaryhepatocytesdifferentiation–Neuronalcelldifferentiation–Endothelialcelltubeformation•CellCultureInsertPlatform–Tumorcellmigration/invasion–Endothelialcellmigration/invasion–Generalcelldifferentiation(3Dco-culture)3Cellculturesystems:2D�3D2D2D�Non-treated(hydrophobic)�BDFalcon™TissueCulture(TC)-treated[net(-)charge]�BDPrimaria™[mixtureof(-)and(+)charge]�BDBioCoat™(largevarietyofECMs,poly-lysine)�BDPureCoat™[carboxyl(-),amine(+);animalfree]3D3D�ECMcoatings(BDMatrigel™matrix,BD™Laminin/Entactin,Collagens)�Rigid3DScaffolds(e.g.,PLA,PGA)�Animal-freehydrogels(BD™PuraMatrix™PeptideHydrogel)�Cellcultureinserts(e.g.,co-culturemodelssuchasBBB)�Animalmodels42Dvs.3DCellCultureYesNoCellEncapsulationSlow;biochemicalgradientsregulatecell-cellcommunicationandsignalingRapidGrowthFactorDiffusion‘Physiological’;promotescloseinteractionsbetweencells,ECMs,growthfactorsNotphysiological;cellspartiallyinteractArchitectureMimicsnaturaltissueenvironmentRigid;inertGrowthSubstrate3D2D5WhyCultureCellsin3D?•Tissueandorgansexistin3D•Studyingcellsin3Denablesresearchersto‘mimic’orapproximatephysiologicalconditionsthatexistinvivo•Stronghistoricalevidencethat3Dcellcultureworksforestablishingdifferentiatedcellmorphologyandfunction__________________________________________________________________FeatureArticlesinNature,August2003Goodbye,FlatBiology?"....biologistsstartingtoexplorethemeritsofculturingcellsin3Dhavebeenstunnedbythedifferenceitmakestothewaycellsbehave,whichismuchclosertotheirbehaviorinvivo."Biology’sNewDimension(highlightsBDMatrigel™matrixandBD™PuraMatrix™)“In10years,anyonetryingtouse2Danalysestogetrelevantandnovelbiologicalinformationwillfinditdifficulttogetfunded.”–Mi...
