第42卷第4期王彤等：美味牛肝菌药用价值及产品开发现状DOI：10.13629/j.cnki.53-1054.2023.04.006中国食用菌圆园23，42（4）：41~46耘阅陨月蕴耘云哉晕郧陨韵云悦匀陨晕粤悦晕缘猿原员园缘源/匝陨杂杂晕员园园猿原愿猿员园贵州2株野生侧耳的鉴定、生物学特性及驯化试验*张秀伟，蔡甫格，吴家丽，鲍菊（安顺市农业科学院，贵州安顺561000）摘要：以2株野生侧耳属菌株ZY-P和SH-P为试验材料，结合形态学与ITS序列分析，构建系统发育树进行鉴定。设定不同因素对菌丝体生物学特性进行研究，筛选出适合菌丝体生长的最佳条件，并通过驯化试验探究野生菌株的出菇特性。结果表明，菌株ZY-P和SH-P分别为糙皮侧耳（Pleurotusostreatus）和哥伦比亚侧耳（Pleurotuscolumbinus）。菌株ZY-P菌丝体生长最佳碳源为葡萄糖，最佳氮源为酵母膏，最适pH为7，最适生长温度为30益，在全黑暗条件下生长最好。菌株SH-P菌丝体生长最佳碳源和氮源分别为甘露醇和酵母膏，最适pH为7~8，最适生长温度为30益，在全光照条件下生长最好。经出菇试验，菌株ZY-P和SH-P第一潮菇生物学效率分别达46.36%、49.89%。关键词：野生侧耳；种属鉴定；生物学特性；驯化中图分类号：S646.1文献标志码：A文章编号：1003-8310（2023）04-0041-06Identification,BiologicalCharacteristicsandDomesticationExperimentofTwoWildStrainsofPleurotusspp.inGuizhouZHANGXiu-wei,CAIFu-ge,WUJia-li,BAOJu(AnshunAcademyofAgriculturalSciences,Anshun561000,China)Abstract:Usingtwowildstrains(ZY-PandSH-P)ofPleurotusspp.asexperimentalmaterials,combinedwithmorphologyandse原quenceanalysis,aphylogenetictreewasconstructedforidentification.Differentfactorsweresettostudythebiologicalcharacteris原ticsofmycelium,andthebestconditionsformyceliumgrowthwereselected.Thefruitingcharacteristicsofthewildstrainswerede原terminedbydomesticationtest.TheresultsshowedthatZY-PandSH-PwereidentifiedasPleurotusostreatusandPleurotuscolumbinusrespectively.ThebestcarbonsourceforZY-Pmyceliumgrowthisglucose,thebestnitrogensourceisyeastextract,thebestpHis7,thebestgrowthtemperatureis30益,andthebestgrowthenvironmentisinfulldarkness.ThebestcarbonandnitrogensourcesforthegrowthofSH-Pmyceliumweremannitolandyeastextract,respectively,theoptimumpHwas7-8,theoptimumgrowthtem...
