分子克隆实验指南 第三版（上、下册）[bioworlde.com].pdfVIP免费

ColdSpringHarborLaboratoryPress-MolecularCloning-TableofContentsTableofContentsChapter1:PlasmidsandTheirUsefulnessinMolecularCloningChapter2:BacteriophageandItsVectorsChapter3:WorkingwithBacteriophageM13VectorsChapter4:WorkingwithHigh-CapacityVectorsChapter5:GelElectrophoresisofDNAandPulsed-FieldAgaroseChapter6:PreparationandAnalysisofEukaryoticGenomicDNAChapter7:Extraction,Purification,andAnalysisofmRNAfromEukaryoticCellsChapter8:InVitroAmplificationofDNAbythePolymeraseChainReactionChapter9:PreparationofRadiolabeledDNAandRNAProbesChapter10:WorkingwithSyntheticOligonucleotideProbesChapter11:PreparationofcDNALibrariesandGeneIdentificationChapter12:DNASequencingChapter13:MutagenesisChapter14:ScreeningExpressionLibrariesChapter15:ExpressionofClonedGenesinEscherichiacoliChapter16:IntroducingClonedGenesintoCulturedMammalianCellsChapter17:AnalysisofGeneExpressioninCulturedMammalianCellsChapter18:ProteinInteractionTechnologiesProtocols|Bioinformatics|Cautions|Trademarks|ForumsContactUs|Help|Logout|MembersHome|MyAccountBuyTheBook|OurVision|TakeTheTour|Newsletter|SearchCopyright©2001byColdSpringHarborLaboratoryPress.Allrightsreserved.Nopartofthesepages,eithertextorimagemaybeusedforanypurposeotherthanpersonaluse.Therefore,reproductionmodification,storageinaretrievalsystemorretransmission,inanyformorbyanymeans,electronic,mechanical,orotherwise,forreasonsotherthanpersonaluse,isstrictlyprohibitedwithoutpriorwrittenpermission.http://www.molecularcloning.com/members/toc.jsp[2002-2-1816:10:23]ColdSpringHarborLaboratoryPress-MolecularCloning-Chapter1Chapter1PlasmidsandTheirUsefulnessinMolecularCloningProtocol1:PreparationofPlasmidDNAbyAlkalineLysiswithSDS:MinipreparationPlasmidDNAisisolatedfromsmall-scale(1-2ml)bacterialculturesbytreatmentwithalkaliandSDS.Protocol2:PreparationofPlasmidDNAbyAlkalineLysiswithSDS:MidipreparationPlasmidDNAisisolatedfromintermediate-scale(20-50ml)bacterialculturesbytreatmentwithalkaliandSDS.Protocol3:PreparationofPlasmidDNAbyAlkalineLysiswithSDS:Maxi...