农产品加工2023年第1期摘要:玉米是啤酒酿造的主要谷物之一,直接添加玉米粉会降低糖化效率,增加啤酒酿造风险,影响啤酒的泡沫性能及风味。采用酶解法,以玉米粉糖化液中的还原糖含量为评价指标,研究了玉米粉糖化工艺。结果表明,最优的糖化工艺条件为料液比1∶5(g∶mL),pH值5.33,耐高温α-淀粉酶Y1的用量42U/g,在90℃下糊化、液化30min,煮沸后加入糖化酶N2124.6U/g,并在60℃下糖化30min。从而可以确定,玉米经过糖化后的糖化液可以直接用于啤酒生产,不仅提高了生产效率,也能提高产品质量。关键词:酶解法;啤酒;玉米粉;糖化;正交试验中图分类号:TS245.5文献标志码:Adoi:10.16693/j.cnki.1671-9646(X).2023.01.010StudyonOptimizationofSaccharificationProcessofCornFlourWENLei1,2,LILi1,LIYuanyi1,*ZONGXuyan1,2(1.CollegeofBiologicalEngineering,SichuanUniversityofScience&Engineering,Yibin,Sichuan644000,China;2.LiquorBrewingBiotechnologyandApplicationKeyLaboratoryofSichuanProvince,SichuanUniversityofScienceandEngineering,Yibin,Sichuan644000,China)Abstract:Cornisoneofthemaingrainsinbeerbrewing.Addingcornflourdirectlywillreducethesaccharificationefficiency,increasetheriskofbeerbrewing,andaffectthefoamperformanceandflavorofbeer.Inthisexperiment,enzymatichydrolysiswasusedtostudythesaccharificationprocessofcornflourwiththecontentofreducingsugarinthesaccharificationsolutionofcornflourastheevaluationindex.Theresultsshowedthattheoptimalsaccharificationprocessconditionswereasfollows:theratioofmaterialtoliquidwas1∶5(g∶mL),thepHofliquidtomaterialwas5.33,theamountofhigh-temperatureresis-tantα-amylaseY1was42U/g,gelatinizedandliquefiedat90℃for30min,boiled,added124.6U/gofsaccharifyingen-zymeN2,andsaccharifiedat60℃for30min.Thus,itcouldbedeterminedthatthesaccharifiedliquidofcornaftersac-charificationcouldbedirectlyusedinbeerproduction,whichnotonlyimprovedproductionefficiencybutalsoimprovedprod-uctquality.Keywords:enzymatichydrolysis;beer;cornflour;saccharification;orthogonalexperiment玉米粉糖化工艺优化研究温磊1,2,李丽1,李远谊1,*宗绪岩1,2(1.四川轻化工大学生物工程学院,四川...