ICS67.100.01X16DB37山东省地方标准DB37/T3531—2019羊奶中羊、大豆源性成分的定性检测方法实时荧光PCR法Identificationofcaprine，sheepandglycinederivedmaterialsingoatorsheepmilkReal-timefluorescentPCRmethod2019-03-21发布2019-04-21实施山东省市场监督管理局发布DB37/T3531—2019I目次前言................................................................................II1范围...............................................................................12规范性引用文件.....................................................................13术语和定义.........................................................................14原理...............................................................................15试剂和材料.........................................................................15.1试剂...........................................................................25.2材料...........................................................................26仪器设备...........................................................................27检测用引物和探针...................................................................38试验步骤...........................................................................38.1取样...........................................................................38.2DNA提取........................................................................38.3实时荧光PCR反应...............................................................39试验数据处理.......................................................................49.1PCR有效性判定..................................................................49.2检测结果分析和表述.............................................................410检测过程中防止交叉污染的措施......................................................4附录A（规范性附录）实时荧光定性PCR引物/探针序列、反应体系和结果判定...........5附录B（资料性附录）目的基因扩增靶标参考序列....................................7DB37/T3531—2019II前言本标准按照GB/T1.1—2009给出...
