ICS65.020.01CCSB36DB52贵州省地方标准DB52/T1528—2020紫苏品种鉴定技术规程SSR标记法Protocolfortheidentificationofperillavarieties—SSRmarkermethod2020-11-13发布2021-03-01实施贵州省市场监督管理局发布DB52/T1528—2020I目次前言................................................................................II1范围..............................................................................12规范性引用文件....................................................................13术语与定义........................................................................14原理..............................................................................15仪器设备及试剂....................................................................26溶液配制..........................................................................27引物信息..........................................................................28参照品种准备......................................................................29操作步骤..........................................................................210数据记录与统计...................................................................311结果判定.........................................................................4附录A（资料性）主要仪器设备及试剂..................................................5附录B（资料性）溶液配制............................................................7附录C（资料性）推荐引物名单及序列..................................................9附录D（资料性）参照品种等位变异位点...............................................11附录E（资料性）参照品种名单.......................................................15DB52/T1528—2020II前言本文件按照GB/T1.1-2020《标准化工作导则第1部分：标准化文件的结构和起草规则》的规定起草。本文件由贵州省农业农村厅提出并归口。本文件起草单位：贵州省油菜研究所。本文件主要起草人：沈奇、温贺、王仙萍、徐静、商志伟、杨森、杜才富、陈俊琨、田飞、汤勇。DB52/T1528—20201紫苏品种鉴定技术规程SSR标记法1范围本文件规定了利用简单重复序列（Simp...
