ICS11.220B41DB37山东省地方标准DB37/T3573—2019牛支原体诊断技术规程CodeofPracticefortheDiagnosisofMycoplasmaBovis2019-05-29发布2019-06-29实施山东省市场监督管理局发布DB37/T3573—2019目次前言..................................................................................II1范围.................................................................................12规范性引用文件.......................................................................13术语与定义...........................................................................14试剂及仪器...........................................................................14.1试剂.............................................................................14.2仪器.............................................................................25样品采集与处理.......................................................................25.1疑似牛支原体感牛只的判断.........................................................25.2疑似病例样品的采集...............................................................25.3样品处理.........................................................................26微生物学检测法.......................................................................26.1液体培养.........................................................................26.2菌落形态观察.....................................................................26.3Dienes染色......................................................................36.4生化试验.........................................................................36.5结果判定.........................................................................37核酸检测法...........................................................................37.1DNA模板的制备...................................................................37.2PCR检测.........................................................................37.3凝胶电泳.........................................................................47.4质量控制......
