研究论文2023年第42卷第1期食品与生物技术学报于晓婷，等：海藻酸钙/硅胶/羧甲基壳聚糖复合微球固定化柚苷酶的研究StudyonImmobilizationofNaringinaseonCalciumAlginate/SilicaGel/CarboxymethylChitosanCompositeMicrospheresYUXiaoting，CHENXiaoyi，LIMiao，WANGShujing，WANGTianyu，LIQian*，LIXianzhen(SchoolofBiologicalEngineering,DalianPolytechnicUniversity,Dalian116034,China)Abstract：Naringinasecanhydrolyzebittersubstances,suchasnaringinincitrusfruits,whichisappliedinjuicedebitteringorpreparationofpharmaceuticalintermediates,forexample,prunin.Inthisstudy,calciumalginate/carboxymethylchitosan/silicagelcompositemicrospheres,whichwereenvironmentalfriendlyandaccessiblewithabundantresources,werepreparedbydroppingmethodandusedascarriersfornaringinaseimmobilization.Theoptimizedspecificenzymeactivity,enzymeloadingrateandenzymeactivityrecoveryofimmobilizednaringinasecouldreach203.33U/g,36.80%and62.15%,respectively,underthefollowingconditions:silicagel/carboxymethylchitosanmassratioof1.0∶1.5,crosslinkingtimeof2.0h,immobilizationtemperatureof25℃,couplingtimeof4h,andenzymedosageof465.6U/mL.Meanwhile,comparedwiththefreenaringinase,thisimmobilizednaringinaseexhibitedbetterstabilitytopHandthermalchanges,andshowedthefeasibilityofoperationandstorage.Besides,theapplicationofnaringinaseimmobilizedoncalcium摘要：柚苷酶可对柑橘类水果中的柚皮苷等苦味物质进行水解，从而实现果汁的脱苦或普鲁宁等医药中间体的制备。作者采用滴入法制备具有环境友好且易获得特性的海藻酸钙/硅胶/羧甲基壳聚糖复合微球，以此作为载体，用于柚苷酶的固定化研究。结果表明，在硅胶/羧甲基壳聚糖质量比1.0∶1.5、交联时间2.0h、固定化温度25℃、偶联时间4h、给酶量465.6U/mL的实验条件下，复合微球固定化柚苷酶的比活力、载酶率和酶活力回收率分别可达203.33U/g、36.80%和62.15%。同时，相比游离柚苷酶，该复合微球固定化柚苷酶对pH和温度变化均显示出更好的稳定性，且更易操作和储藏。此外，海藻酸钙/硅胶/羧甲基壳聚糖复合微球固定化柚苷酶的应用能够进一步拓宽柚苷酶在食品、医药等领域的深入发展。关键词：固定化柚苷酶；复合微球；柚皮苷；果汁脱苦；酶学性质；酶促反应动力...
