第7卷第4期2023年12月现代牧业MODERNANIMALHUSBANDRYVol.7No.4Dec.2023收稿日期:2023-09-16作者简介:何金环(1975—),女,河南郑州人,教授,硕士。聚合草根中尿囊素的提取及其性质分析何金环1,吕敏2,连艳鲜1(1.河南牧业经济学院食品与生物工程学院,河南郑州450046;2.华南农业大学食品学院,广东广州510642)摘要:以聚合草根为原料,采用超声波辅助乙醇法提取尿囊素。以尿囊素得率为考核指标,经单因素和正交优化试验得到的尿囊素提取工艺为乙醇浓度60%、超声功率300W、提取温度60℃,尿囊素得率为0.85mg/g。在0.1~0.3mg/mL浓度范围内,尿囊素对DPPH自由基清除率随尿囊素浓度升高逐渐增加,当尿囊素浓度为0.3mg/mL时,对DPPH清除率达到68%。1mg/mL尿囊素对金黄色葡萄球菌(抑菌直径=39mm)和大肠杆菌(抑菌直径=22mm)的抑菌效果突出,而0.1mg/mL尿囊素几乎没有抑菌作用。关键词:聚合草;尿囊素;提取工艺中图分类号:S816.5文献标识码:A文章编号:2096-2975(2023)04-0032-05ExtractionandcharacterizationofallantoinintherootsofSymphytumofficinaleLHEJinhuan1,LVMin2,LIANYanxian1(1.CollegeofFoodandbioengineeringHenanUniversityofAnimalHusbandryandEconomy,ZhengzhouHenan450046;2.CollegeofFoodScienceSouthChinaAgriculturalUniversity,GuangzhouGuangdong510642)Abstract:AllantoinfromrootsofSymphytumofficinaleLwasextractedbyutrasonicassistedethanolextractionprocess.Takingtheyieldofallantoinastheevaluationindicator,throughsinglefactorandorthogonaloptimizationexperiments,theextractionprocessofallantoinwasobtainedasfollows:ethanolconcentrationof60%,ultrasoundpowerof300W,extrac-tiontemperatureof60℃,andtheyieldofallantoinreached0.85mg/g.Intherangeof0.1to0.3mg/mL,thescaven-gingrateofDPPHfreeradicalsbyallantoingraduallyincreasedwithincreasingconcentrationofallantoin,andwhentheconcentrationofallantoinwas0.3mg/mL,thescavengingratereached68%.1mg/mLallantoinhadaprominentantibac-terialeffectonStaphylococcusaureus(antibacterialdiameter=39mm)andEscherichiacoli(antibacterialdiameter=22mm),while0.1mg/mLallantoinhadalmostnoantibacterialeffect.Keywords:SymphytumofficinaleL;allantoin;extractionprocess尿囊素(allantoin,C4H6O3N4)是嘌呤降解的中间产物,是植物体内氮素存储和转运的重要载体[1,2]。...
