췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍췍本文引文格式:易雪丽,陈晓颖,韦家柱,等.金银花对金黄色葡萄球菌体外抑菌作用研究[J].右江民族医学院学报,2024,46(1):48-51.【论著与临床报道】金银花对金黄色葡萄球菌体外抑菌作用研究易雪丽,陈晓颖,韦家柱,陈启威,庞艳芳(右江民族医学院附属医院检验科,广西百色533000)摘要:目的比较金银花对甲氧西林敏感金黄色葡萄球菌(MSSA)和甲氧西林耐药金黄色葡萄球菌(MRSA)体外抑菌活性差异。方法收集30株MSSA和30株MRSA菌,使用头孢西丁筛选试验和mecA基因检测对菌株进行验证,使用琼脂打孔法检测金银花对两类金黄色葡萄球菌的抑菌活性并比较差异,使用琼脂稀释法检测金银花对两类金黄色葡萄球菌的MIC并计算出MIC50。结果金银花对MSSA和MRSA均有体外抑菌活性且MIC50均为3.125mg/mL。结论金银花对MSSA和MRSA均有较好的体外抑菌活性,在临床治疗金黄色葡萄球菌感染方面有很大的应用前景。关键词:金银花;金黄色葡萄球菌;甲氧西林敏感金黄色葡萄球菌;甲氧西林耐药金黄色葡萄球菌中图分类号:R285文献标识码:A文章编号:1001-5817(2024)01-0048-04doi:10.3969/j.issn.1001-5817.2024.01.009StudyonantibacterialeffectofLoniceraJaponicaThunbonStaphylococcusaureusinvitroYiXueli,ChenXiaoying,WeiJiazhu,ChengQiwei,PangYanfang(DepartmentofClinicalLaboratories,TheAffiliatedHospitalofYoujiangMedicalUniversityforNationalities,Baise533000,Guangxi,China)Abstract:ObjectiveTocomparethedifferenceofantibacterialactivityofLoniceraJaponicaThunba-gainstMethicillin-sensitiveStaphylococcusaureus(MSSA)andMethicillin-resistantStaphylococcusaureus(MRSA)invitro.MethodsThirtystrainsofMSSAandMRSAwerecollectedandidentifiedbyCefoxitinscreeningtestandmecAgenedetection,respectively.Theagarpunchmethodwasusedtodetecttheantibacte-rialactivityofLoniceraJaponicaThunbagainsttwotypesofStaphylococcusaureusandcomparedtheirdiffer-ences.AgardilutionmethodwasusedtodetecttheMICofLoniceraJaponicaThunbagainsttwotypesofStaphylococcusaureus,andtheMIC50wascalculated.ResultsLoniceraJaponicaThunbhadinvitroantibac-terialactivityagainstbothMSSAandMRSAwithMIC50of3.125mg/mL.ConclusionLoniceraJa...
