∗doi:10.3969/j.issn.2095-1736.2023.05.024胶孢炭疽菌侵染秀丽隐杆线虫的特性研究肖月1,易杏盈1,张东华1,刘丽1,闫晓慧1,伍建榕1,2(1.西南林业大学生物多样性保护学院云南省高校森林灾害预警控制重点实验室,昆明650224;2.西南林业大学林学院西南地区生物多样性保育国家林业局重点实验室,昆明650224)摘要通过胶孢炭疽菌(Colletotrichumgloeosporioides)和秀丽隐杆线虫(Caenorhabditiselegans)共培养,探讨该植物病原菌对线虫基本生物学特性的影响。结果显示:胶孢炭疽菌对线虫存活率、体长大小、繁殖能力等基本生物学特性产生显著影响,线虫接触胶孢炭疽菌孢子后存活率显著降低,且虫龄大小与其半数致死时间的长短直接相关,L1期为108h,L4期为144h;胶孢炭疽菌孢子浓度与线虫存活率呈负相关性;与胶孢炭疽菌共培养的线虫体长大小也明显变短,繁殖能力显著降低;然而,胶孢炭疽菌对线虫运动能力和咽泵速率并无显著影响。结果表明:胶孢炭疽菌对秀丽隐杆线虫可产生一定的伤害作用,具有激活线虫天然免疫系统的可能性,该炭疽菌与线虫互作体系可能成为研究天然免疫进化的有力工具。关键词胶孢炭疽菌;秀丽隐杆线虫;存活率;体长大小;繁殖能力中图分类号Q939.95;Q93-337文献标识码A文章编号2095-1736(2023)05-0024-05StudyontheinfectionofCaenorhabditiselegansbyColletotrichumgloeosporioidesXIAOYue1YIXingying1ZHANGDonghua1LIULi1YANXiaohui1WUJianrong121.KeyLaboratoryofForestDisasterWarningandControlinUniversitiesofYunnanCollegeofBiodiversityConservationSouthwestForestryUniversityKunming650224China2.KeyLaboratoryofBiodiversityConservationinSouthwestChinaStateForestryAdministrationCollegeofBiodiversityForestrySouthwestForestryUniversityKunming650224ChinaAbstractTheeffectsoftheplantpathogenonthebasicbiologicalcharacteristicsofCaenorhabditiseleganswerestudiedbyco-cultureofC.elegansandC.gloeosporioides.TheresultsshowedthatC.gloeosporioideshadgreatimpactonthesurvivalbodylengthandre-productiveofC.elegans.ThesurvivalrateofC.elegansdecreasedobviouslyafterexposingtoC.gloeosporioidesandtheageofthewormwasdirectlyrelatedtothehalfdeathtimewhichwas108hinL1stageand144hinL4stagerespectively.Theconidiaconcen-trationofC.gloeosporioidessporeswasnegativelycorrelatedwiththesurvivalofC.elegans.Thebodyl...
