32024,14(2)医师在线第14卷第2期·论著·评价快速碳青霉烯酶灭活试验在肠杆菌科细菌碳青霉烯酶表型检测中的应用价值孙文悦，周小梅，卢金英，梁栋伟（广东省人民医院南海医院检验科，广东佛山528200）【摘要】目的评价快速碳青霉烯酶灭活试验（rCIM）与改良碳青霉烯灭活试验（mCIM）在肠杆菌科细菌碳青霉烯酶表型检测中的应用价值。方法选择86株耐碳青霉烯肠杆菌科细菌，使用聚合酶链式反应（PCR）检测常见碳青霉烯酶基因，比较rCIM与mCIM法检测碳青霉烯酶表型的性能。结果共检测到61株碳青霉烯酶基因型，检出率为70.9%。mCIM法发现阳性菌株61株，阴性菌株25株，rCIM法发现阳性菌株62株，阴性菌株24株。以PCR结果作为金标准，mCIM法的准确度、灵敏度和特异度分别为100.0%、100.0%和100.0%，rCIM法的准确度、灵敏度和特异度分别为98.8%、100.0%和96.0%。结论rCIM法检测肠杆菌科细菌碳青霉烯酶表型，操作简便，不需过夜培养，不需特殊的仪器或试剂，准确度较高。【关键词】肠杆菌科；碳青霉烯酶；碳青霉烯酶灭活试验EvaluationofthevalueofarapidcarbapenemaseinactivationmethodforthephenotypicdetectionofcarbapenemasesinEnterobacteriaceaeSUNWen-yue,ZHOUXiao-mei,LUJin-ying,etal.DepartmentofLaboratoryMedicine,NanhaiHospital,GuangdongProvincialPeople'sHospital,Foshan528200,China.【Abstract】ObjectiveToevaluatetheapplicationvalueofrapidcarbapenemaseinactivationmethod(rCIM)andmodifiedcarbapeneminactivationmethod(mCIM)inthephenotypicdetectionofcarbapenemasesinEnterobacteriaceae.Methods86strainsofcarbapenemresistantEnterobacteriaceaewereselected.ThecommoncarbapenemasegenesweredetectedbyPolymerasechainreaction(PCR).TheperformanceofrCIMandmCIMindetectingcarbapenemasephenotypewascompared.ResultsAtotalof61carbapenemasegenotypesweredetected,withadetectionrateof70.9%.ThemCIMtestfound61positivestrainsand25negativestrains,whiletherCIMtestfound62positivestrainsand24negativestrains.WithPCRresultsasthegoldstandard,thecoincidencerate,sensitivityandspecificityofmCIMtestwere100.0%,100.0%and100.0%,respectively,andthoseofrCIMtestwere98.8%,100.0%and96.0%,respectively.ConclusionTherCIMassaydetectsthecarbapenemasephenotypeofEnterobacteriaceaebacteria,whichiseasytooperate,...
