http://jhc.sagepub.com/JournalofHistochemistry&Cytochemistryhttp://jhc.sagepub.com/content/59/1/13Theonlineversionofthisarticlecanbefoundat:DOI:10.1369/jhc.2010.957191201159:13JHistochemCytochemShan-RongShi,YanShiandCliveR.TaylorTwoDecadesAntigenRetrievalImmunohistochemistry:ReviewandFutureProspectsinResearchandDiagnosisoverPublishedby:http://www.sagepublications.comOnbehalfof:OfficialJournalofTheHistochemicalSocietycanbefoundat:JournalofHistochemistry&CytochemistryAdditionalservicesandinformationforhttp://jhc.sagepub.com/cgi/alertsEmailAlerts:http://jhc.sagepub.com/subscriptionsSubscriptions:http://www.sagepub.com/journalsReprints.navReprints:http://www.sagepub.com/journalsPermissions.navPermissions:WhatisThis?-Feb23,2011VersionofRecord>>byguestonJune2,2012jhc.sagepub.comDownloadedfromJournalofHistochemistry&Cytochemistry59(1)13–32©TheAuthor(s)2011Reprintsandpermission:sagepub.com/journalsPermissions.navDOI:10.1369/jhc.2010.957191http://jhc.sagepub.comJusttwodecadeshavepassedsincethefirstarticleonanti-genretrieval(AR)waspublishedin1991(Shietal.1991).Thissimpletechniqueofboilingformalin-fixedparaffin-embedded(FFPE)tissuesectionsinwaterhasplayedamajorroleinextendingthereachanduseofimmunohisto-chemistry(IHC)inFFPEtissues(Gownetal.1993;TaylorandCote2005).OnenotableresultistheeffectivedivisionofallpublicationswithrespecttoIHCforFFPEtissuesec-tionsintotwoeras:pre-ARandpost-AR(Gown2004;Tay-lor2001),indicatingARasamilestone(Jagirdar2008).Sincetheearly1950s,theJournalofHistochemistry&Cytochemistryhaspublishednumerousarticlesdescribingnewandinterestingtechniquesformorphologicexamina-tionoftissues,suchastheenzyme-labeledIHC(NakaneandPierce1966),avidin-biotindetectionsystem(Hsuetal.1981),AR(Shietal.1991;Shietal.1992),tyramidesignalamplification(Adams1992),andsoon.Allofthesevalu-abledevelopmentshavecontributedtoclinicalandbasicbiomedicalresearchprojectsworldwide.AnumberoffactorscontributetothemajorimpactthatARhashadondiagnosticpathology(BoonandKok1995;Dabbs2008;Jagirdar200...
