www.wjgnet.comwcjd@wjgnet.com世界华人消化杂志2010年4月28日;18(12):1232-1236ISSN1009-3079CN14-1260/R临床研究CLINICALRESEARCHMAL基因在胃癌组织中的甲基化及其mRNA的表达林若阳,沈萍萍,黄智铭,郑建建,王良兴林若阳,沈萍萍,黄智铭,郑建建,王良兴,温州医学院附属第一医院浙江省温州市325000林若阳,2007级温州医学院硕士,主要从事消化系恶性肿瘤侵袭转移的基础和临床研究.作者贡献分布:此课题由林若阳设计;研究过程由林若阳、沈萍萍及郑建建完成;数据分析由林若阳与沈萍萍完成;本论文写作由林若阳完成;黄智铭对文章内容作批评性审阅;黄智铭与王良兴提供本实验经费.通讯作者:黄智铭,主任医师,325000,浙江省温州市,温州医学院附属第一医院消化内科.wyyyhzhiming@126.com电话:0577-88069256收稿日期:2009-12-31修回日期:2010-02-15接受日期:2010-02-23在线出版日期:2010-04-28MALmRNAexpressionandDNAmethylationingastriccancerRuo-YangLin,Ping-PingShen,Zhi-MingHuang,Jian-JianZheng,Liang-XingWangRuo-YangLin,Ping-PingShen,Zhi-MingHuang,Jian-JianZheng,Liang-XingWang,DepartmentofGastroen-terology,theFirstAffiliatedHospitalofWenzhouMedicalCollege,Wenzhou325000,ZhejiangProvince,ChinaCorrespondingto:Zhi-MingHuang,DepartmentofGas-troenterology,theFirstAffiliatedHospitalofWenzhouMedicalCollege,Wenzhou325000ZhejiangProvince,China.wyyyhzhiming@126.comReceived:2009-12-31Revised:2010-02-15Accepted:2010-02-23Publishedonline:2010-04-28AbstractAIM:ToinvestigatethemRNAexpressionandDNAmethylationoftheMALgeneingastriccarcinoma.METHODS:Methylation-specificPCR(MSP)andreal-timereversetranscription-polymerasechainreaction(RT-PCR)wereusedtodetectDNAmethylationandmRNAexpressionoftheMALgenein37gastriccarcinomasamplesandmatchedtumor-adjacenttissuesamples,respec-tively.ThecorrelationsofDNAmethylationandmRNAexpressionoftheMALgenewithclinicalandpathologicalparametersingastriccarcino-mawerethenanalyzed.RESULTS:ThemethylationrateoftheMALgeneis78.4%(29/37)ingastriccarcinomatissueand5.4%(2/37)intumor-adjacenttissue,withasignificantdifferencebetweenthetwogroups(P<0.01).MALgenemethylationisnotassociatedwithage,sex,tumorsize,lymphnodemetasta-sis,tumordifferentiationa...
