INSTRUCTIONSPierceBiotechnologyPOBox117(815)968-0747www.thermoscientific.com/pierce3747N.MeridianRoadRockford,lL61105USA(815)968-7316faxNumberDescriptionEH3TNFAHumanTumorNecrosisFactorα(TNFα)ELISAKit,sufficientreagentsfor96determinationsEH3TNFA2HumanTumorNecrosisFactorα(TNFα)ELISAKit,sufficientreagentsfor2×96determinationsEH3TNFA5HumanTNFαELISAKit,sufficientreagentsfor5×96determinationsKitContentsEH3TNFAEH3TNFA2EH3TNFA5Anti-humanTNFαPrecoated96-wellStripPlate1each2each5eachLyophilizedRecombinantHumanTNFαStandard2vials4vials5vialsSampleDiluent,contains0.1%sodiumazide26mL2×26mL75mL30XWashBuffer50mL2×50mL200mLBiotinylatedAntibodyReagent,contains0.1%sodiumazide13mL2×13mL56mLStreptavidin-HRPReagent14mL2×14mL70mLTMBSubstrate13mL2×13mL5×13mLStopSolution,contains0.16Msulfuricacid13mL2×13mL55mLAdhesiveplatesealers6each12each30eachForresearchuseonly.Notforuseindiagnosticprocedures.Storage:Formaximumstability,storeinanon-defrosting-20ºCfreezerandrefertotheexpirationdateforfrozenstorageonthelabel.Alternatively,storeat2-8ºCandrefertotheexpirationdateforrefrigeratedstorage.Oncethawed,storeat4ºCuntiltheexpirationdateforrefrigeratedstorage.Kitisshippedondryice.TableofContentsIntroduction.................................................................................................................................................................................1ProcedureSummary.....................................................................................................................................................................2AdditionalMaterialsRequired.....................................................................................................................................................2Precautions...................................................................................................................................................................................2SamplePreparation..............................................
