JournalofVisualizedExperimentswww.jove.comCopyright©2011CreativeCommonsAttribution-NonCommercialLicensePage1of1MaterialsListfor:DetectionofPost-translationalModificationsonNativeIntactNucleosomesbyELISABoDai1,FaridaDahmani2,JosephA.Cichocki3,LindseyC.Swanson2,TheodoreP.Rasmussen31InstituteforStemCellBiologyandRegenerativeMedicine,StanfordUniversity2DepartmentofMolecularandCellBiology,UniversityofConnecticut3DepartmentofPharmaceuticalSciences,UniversityofConnecticutCorrespondenceto:TheodoreP.Rasmussenattheodore.rasmussen@uconn.eduURL:http://www.jove.com/video/2593DOI:doi:10.3791/2593MaterialsNameCompanyCatalogNumberCommentsMicrococcalNucleaeSigma-AldrichN3755Makealiquotsof2U/10μlbuffer,storedat-20°CNuncMaxiSorpMicrotiterPlatesFisherScientific12-565-135AdhesiveplatecoverscanalsobeorderedthroughFisherAutomatedPlateWasher1-StepTMB-ELISAsubstratePierce,ThermoScientific34028Storeat4°Cpre-warmtoRTbeforeuseorasdirectedonthespecificationsheetMicrotiterPlateReaderBio-RadMostcolorimetricplatereadermodelsaresuitable
