JournalofVisualizedExperimentswww.jove.comCopyright©2011JournalofVisualizedExperimentsandCorning,Inc.Page1of1MaterialsListfor:Scale-UpofMammalianCellCultureusingaNewMultilayeredFlaskElizabethJ.Abraham1,KatieA.Slater1,SuparnaSanyal1,KenLinehan1,PaulaM.Flaherty1,SusanQian11BDBiosciencesURL:http://www.jove.com/video/3418DOI:doi:10.3791/3418MaterialsNameCompanyCatalogNumberComments3-layerTissueCulture-treated525cm2BDBiosciences3531435-layerTissueCulture-treated875cm2BDBiosciences353144100mlpipetteBDBiosciences35760010mlpipetteBDBiosciences3575515mlaspiratingpipetteBDBiosciences35750150mlPolypropyleneconicaltubeBDBiosciences352070T-175flaskTissueCulture-treatedBDBiosciences353028GramCrystalVioletBDBiosciences212525DMEMInvitrogen11885GMEMSigma-AldrichG5154RPMI-1640ATCC30-2001MEMATCC30-2003Trypsin-EDTALonzaInc.CC-5012FetalBovineserumInvitrogen16000-044TryptosePhosphateBrothSigma-AldrichT8159BHK-21cellsSigma-Aldrich85011433Hep-G2,LnCAPATCCEcopack2-293ClontechLaboratoriesParaformaldehydeElectronMicroscopySciences15710PolystyreneBeadPolysciences,Inc.24628-20Bio-ProfileFlexAnalyzerNovaBioMedical
