SPC-354250-Grev3.0BD™PuraMatrix™PeptideHydrogelCatalogNo.354250GuidelinesforUseFORRESEARCHUSEONLY.NOTFORCLINICAL,DIAGNOSTIC.ORTHERAPEUTICPROCEDURES.NOTFORUSEINHUMANS.BDBiosciences–DiscoveryLabwareTwoOakPark,Bedford,MA01730,tel:877.232.8995,fax:800.325.9637,bdbiosciences.comForResearchUseOnly.Notforuseinclinical,diagnostic,ortherapeuticprocedures.Notforuseinhumans.1SPC-354250-Grev4.0TABLEOFCONTENTSPageINTENDEDUSE....................................................................................................................................2MATERIALSPROVIDED......................................................................................................................3SUPPLEMENTAL/OPTIONALMATERIALS(NOTSUPPLIED).........................................................3PRECAUTIONS.....................................................................................................................................3GENERALUSE.....................................................................................................................................4PROCEDURESFORUSE....................................................................................................................4A)SurfacePlatingofAdherentCellsinCellCulturePlates...................................................................4B)SurfacePlatingofAdherentCellsinCellCultureInsert.....................................................................5C)ECMPreparationforSupplementationofBD™PuraMatrix™PeptideHydrogel...............................6D)3DCellEncapsulationinCellCulturePlates(withorwithoutECMproteinsupplementation)........8E)3DCellEncapsulationinCellCultureInserts(withorwithoutECMproteinsupplementation).......8F)CellDropCultureProtocol...............................................................................................................11G)CellRecoveryforSub-CulturingorBiochemicalAnalyses............................................................12H)FluorescentStaining...
