Poly-L-lysinesolutionCellCultureTestedProductNumberP4707StorageTemperature2-8°CProductDescriptionCASNumber:25988-63-0Molecularweightrange:70-150kDaThisproductissuppliedasa0.01%sterile-filteredsolutioninwaterandistestedforuseincellcultureapplications.Poly-L-Lysineisachargeenhancer,therefore,itcanbeusedforcoatingmanysurfaces.WhenusedtocoatELISAplates,ittendstoimprovebindingofIgG.HighpHwillaidbindingtotheplates.Certaincellscandigestpoly-L-lysine,inwhichcasethepoly-D-lysineshouldbeused.Thelowermolecularweightpoly-L-lysine(30-70kDa)iseasiertousebecauseitislessviscousinsolution,butthehighermolecularweightpoly-L-lysine(>300kDa)providesmoreattachmentsitespermolecule.Themid-rangeproducthasaMWrangeof70-150kDa.Theremightbeasmallamountofproductintheβstructure,butverylittle.ThisisbecausetheHBrinterfereswithhydrogenbondingbetweentheaminoandcarboxylgroups,orbetweentheaminogroupandotherNorOcontainingmoieties.Thesecondarystructuresofpolyaminoacidshaveneverbeenextensivelystudied,butitisknownthatthedegreeofsecondaryconformationalregularitydependsontheaminoacidinthepolymer.PrecautionsandDisclaimerForLaboratoryUseOnly.Notfordrug,householdorotheruses.Storage/StabilityTransitionofahighmolecularweightpoly-L-lysine(degreeofpolymerization1,500)inaqueoussolutionfromahelicaltoarandomcoiledconformationoccursundertheinfluenceofdecreasingpHorincreasingtemperature.1ProcedureForcoatingslideswithpoly-L-lysine,ingeneral,itisrecommendedtousea0.01%(w/v)solution.Aftera5minuteincubation,theexcesssolutionisremovedandtheslidesaredriedatroomtemperatureorinanovenatgentleheat.Coatedslidesshouldbestableforoneyearifprotectedfromdust.Thereisnoneedtosterilzeslidescoatedwithpoly-L-lysinebyautoclaving;simplyexposetheslidestoUVlightovernight.Ifunevencoatingoccurs,theglassslidesmaybepretreatedwith1mMmagnesiumacetatefor2-3hoursandthenrinsedwellbeforecoating.Alternatively,theymaybeacidwashed(hydrochloricacidorsulfuricacid).Thistreatmentshouldallowforanevencoatingwiththepoly-L-lysinesolution.Thisproductcanalsobeusedtocoattissuecultureplasticware.Asolutionof0.1mg/mlispreparedand0.5mlisasepticallytransferredtoanon-tissueculturetreated25cm2flask.Theflaskisgentlyrockedtoevenlycoatthesurface.Afterfiveminutes,theexcesssolutionisremovedandthesurfaceisthoroughlyrinsedwithsterilewaterandallowedtodryforseveralhours.References1.TheMerckIndex,11thed.,Entry#7549.CMH/RXR12/04SigmabrandproductsaresoldthroughSigma-Aldrich,Inc.Sigma-Aldrich,Inc.warrantsthatitsproductsconformtotheinformationcontainedinthisandotherSigma-Aldrichpublications.Purchasermustdeterminethesuitabilityoftheproduct(s)fortheirparticularuse.Additionaltermsandconditionsmayapply.Pleaseseereversesideoftheinvoiceorpackingslip.
