JournalofVisualizedExperimentswww.jove.comCopyright©2012CreativeCommonsAttribution-NonCommercialLicenseMarch2012|61|e3721|Page1of5VideoArticleDetectionofInvasivePulmonaryAspergillosisinHaematologicalMalignancyPatientsbyusingLateral-flowTechnologyChristopherThornton1,GemmaJohnson2,SamirAgrawal31Biosciences,UniversityofExeter2BICMS,QueenMaryUniversityofLondon3St.Bartholomew'sHospitalandTheLondonNHSTrustCorrespondenceto:ChristopherThorntonatC.R.Thornton@ex.ac.ukURL:http://www.jove.com/video/3721DOI:doi:10.3791/3721Keywords:Immunology,Issue61,Invasivepulmonaryaspergillosis,acutemyeloidleukemia,bonemarrowtransplant,diagnosis,monoclonalantibody,lateral-flowtechnologyDatePublished:3/22/2012Citation:Thornton,C.,Johnson,G.,Agrawal,S.DetectionofInvasivePulmonaryAspergillosisinHaematologicalMalignancyPatientsbyusingLateral-flowTechnology.J.Vis.Exp.(61),e3721,doi:10.3791/3721(2012).AbstractInvasivepulmonaryaspergillosis(IPA)isaleadingcauseofmorbidityandmortalityinhaematologicalmalignancypatientsandhematopoieticstemcelltransplantrecipients1.DetectionofIPArepresentsaformidablediagnosticchallengeand,intheabsenceofa'goldstandard',reliesonacombinationofclinicaldataandmicrobiologyandhistopathologywherefeasible.DiagnosisofIPAmustconformtotheEuropeanOrganizationforResearchandTreatmentofCancerandtheNationalInstituteofAllergyandInfectiousDiseasesMycologyStudyGroup(EORTC/MSG)consensusdefining"proven","probable",and"possible"invasivefungaldiseases2.Currently,nonucleicacid-basedtestshavebeenexternallyvalidatedforIPAdetectionandsopolymerasechainreaction(PCR)isnotincludedincurrentEORTC/MSGdiagnosticcriteria.IdentificationofAspergillusinhistologicalsectionsisproblematicbecauseofsimilaritiesinhyphalmorphologieswithotherinvasivefungalpathogens3,andprovenidentificationrequiresisolationoftheetiologicagentinpureculture.Culture-basedapproachesrelyontheavailabilityofbiopsysamples,butthesearenotalwaysaccessibleinsickpatients,anddonotalwaysyieldviablepropagulesforculturewhenobtained.Animportantfeat...
