JournalofVisualizedExperimentswww.jove.comCopyright©2011JournalofVisualizedExperimentsPage1of1MaterialsListfor:OptimizedProtocolforEfficientTransfectionofDendriticCellswithoutCellMaturationRobertBowles*1,SonaliPatil*1,HannaPincas1,StuartC.Sealfon11CenterforTranslationalSystemsBiologyandDepartmentofNeurology,MountSinaiSchoolofMedicine*TheseauthorscontributedequallyCorrespondenceto:StuartC.Sealfonatstuart.sealfon@mssm.eduURL:http://www.jove.com/video/2766DOI:doi:10.3791/2766MaterialsNameCompanyCatalogNumberCommentsAmaxaNucleofector96-wellShuttleLonzaInc.108S0109SerialnumberAmaxaHumanMonocyte96-wellNucleofectorKitLonzaInc.VHPA-2007ContainstheHumanMonocyte96-wellNucleofectorSolution,the96-wellSupplementandtheNucleocuvettesandplatesRIG-IsiRNADharmaconL-012511-00GLOsiRNADharmaconD-001600-01-20RPMI1640Invitrogen11875Supplementedwith10%FCS,2mML-glutamine,100U/mlpenicillinand100μg/mlstreptomycintomakeDCgrowthmediumDMEMInvitrogen11965L-glutamineInvitrogen25030081Penicillin/streptomycinInvitrogen15070063FetalCalfSerumHyclone3070.03DendriticCellsNewYorkBloodcenterDCsarepurifiedfrombuffycoatsusingastandardprocedure
