JournalofVisualizedExperimentswww.jove.comCopyright©2011CreativeCommonsAttribution-NonCommercialLicensePage1of1MaterialsListfor:ACalciumBioluminescenceAssayforFunctionalAnalysisofMosquito(Aedesaegypti)andTick(Rhipicephalusmicroplus)GProtein-coupledReceptorsHsiao-LingLu1,CymonN.Kersch1,SuparnaTaneja-Bageshwar1,2,PatriciaV.Pietrantonio11DepartmentofEntomology,TexasA&MUniversity(TAMU)2DepartmentofMolecularandCellularMedicine,TexasA&MUniversity(TAMU)Correspondenceto:PatriciaV.Pietrantonioatp-pietrantonio@tamu.eduURL:http://www.jove.com/video/2732DOI:doi:10.3791/2732MaterialsNameCompanyCatalogNumberCommentsDMEMInvitrogenABCD1234CHO-K1cellsATCCCCL-61Manassas,VA,USAF12KmediumInvitrogen21127FetalBovineSerumSigma-AldrichF0643Trypsin-EDTA(10x)Invitrogen15400Antibiotic-AntimycoticInvitrogen15240Opti-MEMIReducedSerumMediumInvitrogen31985LipofectinReagentInvitrogen18292-011GENETICINInvitrogen10131035MC1061/P3UltracompInvitrogenC663-03QIAprepspinminiprepkitQiagen19064FuGENE6TransfectionreagentRocheGroup1181444300196-wellwhitethinbottommicrotitereplateCostar3610calcium-freeDMEMmediaInvitrogen21068C–lenterazineInvitrogenC-2944Bright-LineHemacytometerHausserScientificHorsham,PANOVOstarBMGLabtechnologiesPrismsoftware4.0GraphPadSoftwareInc.SanDiego,CA,USAT-25andT-75FlasksBDBiosciences353014and353135
