JournalofVisualizedExperimentswww.jove.comCopyright©2012JournalofVisualizedExperimentsPage1of1MaterialsListfor:Genome-wideGeneDeletionsinStreptococcussanguinisbyHighThroughputPCRXiuchunGe1,PingXu11ThePhilipsInstituteofOralandCraniofacialMolecularBiology,VirginiaCommonwealthUniversityCorrespondenceto:PingXuatpxu@vcu.eduURL:http://www.jove.com/video/4356DOI:doi:10.3791/4356MaterialsNameCompanyCatalogNumberCommentsPrimerF2IntegratedDNATechnologiesTGACTAACTAGGAGGAATAAATGGCTAAAATGAGAATATPrimerR2ibidCATTATTCCCTCCAGGTACTAAAACAATTCATCCAGTPrimerF2pibidGATAAACCCAGCGAACCATTTGAPrimerP1ibidGCTTATATACCTTAGCAGGAGACAPrimerP2ibidGTATGACATTGCCTTCTGCGTCCPrimer5'end_R1_seqibidGCCATTTATTCCTCCTAGTTAGTCAPrimer5'end_F3_seqibidGTTTTAGTACCTGGAGGGAATAATGPrimer5'end_R1p_seqibidCCTCAAATGGTTCGCTGGGTTTATCCSPSynprepSequence:NH2-DLRGVPNPWGWIFGR-COOHDNAPolymeraseInvitrogen11304-102PlatinumTaqDNAPolymeraseHighFidelityEcoRINewEnglandBiolabsIncR0101SRestrictionenzymeAgarAmericanBioanalyticalAB01185AgarosePromegaV3125BHIBDBiosciences237500BrainHeartInfusionHorseserumFisherScientificSH3007403HorseserumKmFisherScientificBP906-5KanamycinTHbrothBDBiosciences249240ToddHewittbrothPureLink96InvitrogenK310096PureLink96PCRpurificationkitQIAquickQiagen28106QIAquickPCRpurificationkitFilterCorning4311170.22μmpolystyrenefilterAnoxomatSystemMartMicrobiologyb.v.AnoxomatMarkIIBenchtopcentrifugeThermoScientific75004377SorvallLegendRTPlusmicroplaterotorGelDocumentationUVPLLCBioDoc-It210IncubatorFisherScientific11-690-625DIsotempLabnet'sGelXLLabnetE0160Labnet'sGelXLMicrocentrifugeEppendorf22621408Microcentrifuge5415RMultichannelpipettesThermoScientfic4661040,4661020FinnpipetteF1ThermalCyclerAppliedBiosystemsInc.N805-0200GeneAmpPCRsystem9700
