JournalofVisualizedExperimentswww.jove.comCopyright©2012CreativeCommonsAttribution-NonCommercialLicensePage1of1MaterialsListfor:PreparationofMouseEmbryonicFibroblastCellsSuitableforCulturingHumanEmbryonicandInducedPluripotentStemCellsJustynaJozefczuk1,KatharinaDrews1,JamesAdjaye11DepartmentofVertebrateGenomics,MaxPlanckInstituteforMolecularGeneticsCorrespondenceto:JustynaJozefczukatjagodzin@molgen.mpg.de,JamesAdjayeatadjaye@molgen.mpg.deURL:http://www.jove.com/video/3854DOI:doi:10.3791/3854MaterialsNameCompanyCatalogNumberCommentsDMEM(HighGlucose)Gibco,Invitrogen41966-052FBSBiochromS0115L-glutamineGibco,Invitrogen25030-024Penicillin-streptomycinGibco,Invitrogen15140-122VacuumfiltersystemCorning431097500ml,0.22μm,PASDMSOSigmaD2650KnockoutDMEMGibco,Invitrogen10829-018KnockoutSerumReplacementGibco,Invitrogen10828-028Non-EssentialAminoAcidsGibco,Invitrogen11140-035beta-Mercapt–thanolSigmaM7522PBSGibco,Invitrogen14190-169DNaseISigmaD4527MitomycinCRoche10107409001BasicFibroblastGrowthFactorPeproTech100-18BBiotinylatedAnti-human/mouse/ratActivinAAntibodyR&DSystemsBAM3381GelatinSigmaG9391Human/Mouse/RatActivinAMAbR&DSystemsMAB33810.05%Trypsin-EDTAGibco,Invitrogen25300-054ExtraThinIrisScissorsFST14088-10ExtraFineGraefeForcepsFST11150-10PierseFixationForcepsFST18155-13
