JournalofVisualizedExperimentswww.jove.comCopyright©2011JournalofVisualizedExperimentsPage1of1MaterialsListfor:ExtractionofHighMolecularWeightDNAfromMicrobialMatsBenjaminS.Bey1,ErinB.Fichot1,R.SeanNorman11DepartmentofEnvironmentalHealthSciences,ArnoldSchoolofPublicHealth,UniversityofSouthCarolinaCorrespondenceto:R.SeanNormanatrsnorman@mailbox.sc.eduURL:http://www.jove.com/video/2887DOI:doi:10.3791/2887MaterialsNameCompanyCatalogNumberCommentsβ-mercapt–thanolSigma-AldrichM3148Polyethyleneglycol8000PromegaCorp.V301120%in1.2MNaClPotassiumacetateFisherScientificFisherScientificQuant-iTdsDNAAssaykitInvitrogenQ33130RNaseEpicentreBiotechnologiesMRNA092SodiumChlorideVWRBDH8014Appropriateconc.SodiumDodecylSulfateFisherScientific03-500-50910%inwatersodiumhexametaphosphateEMDMilliporeSX0583-32%inwaterTBEFisherScientificBP1333-1CHEFMapperXASystemBio-Rad170-3670NanoDrop1000spectrophotometerThermoFisherScientific,Inc.ND-1000VortexerScientificIndustriesInc.UltravioletCrosslinkerUVPInc.WaringblenderWaringLaboratoryLB10S
