JournalofVisualizedExperimentswww.jove.comCopyright©2011JournalofVisualizedExperimentsPage1of1MaterialsListfor:ProteinMembraneOverlayAssay:AProtocoltoTestInteractionBetweenSolubleandInsolubleProteinsinvitroShokoUeki1,BenoîtLacroix1,VitalyCitovsky11DepartmentofBiochemistryandCellBiology,StateUniversityofNewYorkCorrespondenceto:ShokoUekiatsueki@ms.cc.sunysb.eduURL:http://www.jove.com/video/2961DOI:doi:10.3791/2961MaterialsNameCompanyCatalogNumberCommentsProteinassaykitBio-Rad500-0001ProteinaseinhibitorcocktailSigma-AldrichS8820Mini-PROTEANsystemBio-Rad165-8000Semi-drywesternblottingSDelectrotransfersystemBio-Rad170-3940Anti-rabbitIgGantibodyconjugatedwithhorseradishperoxidaseGenScriptA00098Anti-GSTrabbitpolyclonalantibodyGenScriptA00097Anti-strepIIGenScriptA00626BioTrace,NTnitrocellulosetransfermembranePallCorporation27377-000ImmobilonwesternchemiluminescentHRPsubstrateEMDMilliporeWBKLS0050
