www.roche-applied-science.comLightCycler®480HighResolutionMeltingMasterVersionFebruary2008Easy-to-useReactionMix(2�conc.)forPCRandhighresolutionmeltingusingtheLightCycler®480Real-TimePCRSystemCat.No.04909631001Kitfor5�100reactions(20�l)Storethekitat�15to�25°CNKeeptheMasterMix(vial1)awayfromlight.Forgenerallaboratoryuse.Notforuseindiagnosticprocedures.FORINVITROUSEONLY.TableofContents2www.roche-applied-science.com1.WhatthisProductDoes.................................................................................................1NumberofTests1KitContents1StorageandStability1AdditionalEquipmentandReagentsRequired2Application2AssayTime32.HowtoUsethisProduct................................................................................................42.1BeforeYouBegin4SampleMaterial4Primers4MgCl25NegativeControl52.2Procedure6LightCycler®480SystemProtocol6PreparationofthePCRMix82.3RelatedProcedures9PreventionofCarry-OverContamination93.Results.............................................................................................................................104.Troubleshooting............................................................................................................125.AdditionalInformationonthisProduct....................................................................14HowthisProductWorks14TestPrinciple14References15QualityControl156.SupplementaryInformation........................................................................................166.1Conventions166.1.1TextConventions166.1.2Symbols166.2ChangestoPreviousVersion166.3OrderingInformation176.4DisclaimerofLicense196.5Trademarks19PROTOCOLPROTOCOLwww.roche-applied-science.com1WhatthisProductDoes1.WhatthisProductDoesNumberofTestsThekitisdesignedfor5�100reactionswithareactionvolumeof20�leach.KitContentsStorageandStabilityLThekitisshippedondryice.•Ifstoredat�15to�25°Cthekitisstablethroughtheexpirationdateprintedonthelabel.•Oncethekitisopened,storethekitcomponentsasdescr...
