JournalofVisualizedExperimentswww.jove.comCopyright©2012JournalofVisualizedExperimentsPage1of1MaterialsListfor:ModifiedYeast-Two-HybridSystemtoIdentifyProteinsInteractingwiththeGrowthFactorProgranulinQing-YunTian1,Yun-PengZhao1,Chuan-juLiu1,21DepartmentofOrthopaedicSurgery,NYUHospitalforJointDiseases2DepartmentofCellBiology,NewYorkUniversitySchoolofMedicineCorrespondenceto:Chuan-juLiuatchuanju.liu@med.nyu.eduURL:http://www.jove.com/video/3562DOI:doi:10.3791/3562MaterialsNameCompanyCatalogNumberCommentsProQuesttwo-hybridsystemInvitrogen10835YPDGrowthMediumClontechLaboratories630409YPDAgarMediumClontechLaboratories630410MinimalSDagarBaseClontechLaboratories630412-LeuDOSupplementClontechLaboratories630414-Leu/-TrpDOSupplementClontechLaboratories630417-His/-Leu/-Trp/-UraDOSupplementClontechLaboratories6304253-Amino-1,2,4-Triazole(3AT)Sigma-AldrichA8056Luriabroth(LB)Sigma-AldrichL3022X-GalInvitrogen15520-034SonicatedSalmonSpermDNAStratagene,AgilentTechnologies201190AmpicillinAMRESCO0339KanamycinSulfateInvitrogen11815-024SubcloningEfficiency™DH5α™CompetentCellsInvitrogen18265-017Trizma®baseSigma-AldrichT6066LithiumacetateSigma-AldrichL4158EthylenediaminetetraaceticacidSigma-AldrichEDNitrocelluloseMembraneBio-Rad162-011510-cmpetridishITIScientificCT-903Incubator(30°C)ATR(Ecotron)
