JournalofVisualizedExperimentswww.jove.comCopyright©2013JournalofVisualizedExperimentsPage1of1MaterialsListfor:WholeMountRNAFluorescentinsituHybridizationofDrosophilaEmbryosFélixLegendre*1,2,NealCody*1,CaroleIampietro1,JulieBergalet1,FabioAlexisLefebvre1,2,GaëlMoquin-Beaudry1,2,OliviaZhang1,XiaofengWang1,EricLécuyer1,21InstitutdeRecherchesCliniquesdeMontréal(IRCM)2DepartmentofBiochemistry,UniversitédeMontréal*TheseauthorscontributedequallyCorrespondenceto:EricLécuyeratEric.Lecuyer@ircm.qc.caURL:http://www.jove.com/video/50057DOI:doi:10.3791/50057MaterialsNameCompanyCatalogNumberCommentsT7,T3orSP6RNAPolymeraseFermentasLifeSciencesEP0101,EP0111,EP0131Kitscontainreactionbuffer.DIGRNALabelingMixRocheAppliedScience11277073910RNAguardAmershamBiosciences27-0816-013MsodiumacetateCold100%ethanol.Cold70%ethanol.Chlorinebleachsolutiondiluted1:1withwater.HeptaneMethanolproteinaseKSigmaAldrichOakville,ON,CanadaCatalogNo.P230840%formaldehydesolution,freshlypreparedPBS-Tweensolution(PBT)1xPBS,0.1%Tween-20Glycinesolution2mg/mlglycineinPBTHRP-conjugatedmousemonoclonalanti-DIGJacksonImmunoResearchLaboratoriesInc200-032-156(1/400dilutionofa1mg/mlstocksolutioninPBTBHRP-conjugatedsheepmonoclonalanti-DIGRocheAppliedScience,Laval,QC12077331/500dilutionofstocksolutioninPBTBBiotin-conjugatedmousemonoclonalanti-DIGJacksonImmunoResearchLaboratoriesInc.,WestGrove,PA,USA200-062-156(1/400dilutionofa1mg/mlstocksolutioninPBTBStreptavidin-HRPconjugateMolecularProbes,EugeneOR,USAS991(1/100dilutionofa1μg/mlstock
