JournalofVisualizedExperimentswww.jove.comCopyright©2009JournalofVisualizedExperimentsPage1of2MaterialsListfor:LargeInsertEnvironmentalGenomicLibraryProductionMarcusTaupp1,SangwonLee1,AlyseHawley1,JinshuYang1,StevenJ.Hallam11DepartmentofMicrobiologyandImmunology,UniversityofBritishColumbia-UBCCorrespondenceto:StevenJ.Hallamatshallam@interchange.ubcURL:http://www.jove.com/video/1387DOI:doi:10.3791/1387MaterialsNameCompanyCatalogNumberCommentsStepIICopyControl™FosmidLibraryProductionKitEpicentreBiotechnologiesCCFOS110sufficienttogenerate9-10fosmidlibrariesSeaPlaqueAgaroseCambrex50100stirringhotplateCorningPC-420D1.0XTAErunningbufferCHEFMapperXApulsedfieldelectrophoresissystemincludingcoolingmoduleandvariablespeedpumpBio-RadλDNA-HindIIIDigestNewEnglandBiolabsN3012SMidRangeIPFGMarkerNewEnglandBiolabsN3551S10,000XSYBRGoldInvitrogenS11494microwavableplasticwrapSaranpremiumwrapSafeImagertransilluminatorInvitrogenS37102GELaseAgaroseGel-DigestingPreparationEpicentreBiotechnologiesG09100scalpelFisherScientific08-927-5DAmiconUltra-4CentrifugalFilterUnitwithUltracel-10membraneEMDMilliporeUFC801024MicroconYM-30CentrifugalFilterUnitEMDMillipore42410nucleasefreewaterAmbionAM9932Quant-iTPicoGreendsDNAAssayKit*2000assays*#InvitrogenP7589digitaldryblockheaterVWRinternational12621-088waterbathVWRinternational14231-854centrifugeBeckmanCoulterInc.AvantiJ-EcentrifugerotorBeckmanCoulterInc.JA5.3tabletopcentrifugeEppendorf5415Dmicrocentrifugetubes,1.7mL,clearAxygenScientificMCT-175-C15mLcentrifugetubesFisherScientific430052StepIIILBbrothFisherScientificBP1426-2MgSO4Sigma-AldrichM2643phagedilutionbuffer10mMTris-HCl[pH8.3],100mMNaCl,10mMMgCl2JournalofVisualizedExperimentswww.jove.comCopyright©2009JournalofVisualizedExperimentsPage2of2cryogenicvialsFisherScientific430488StepIV96wellplatewithlidFisherScientificCS003370(3370)384wellplatewithlidFisherScientific07201157(3680)Petridishes100O.D.x15mmHFisherScientific08-757-12Petridishes150Dia.x15mmHFisherScientific08-757-14BDFalconBioDishXL245X245mmVWRinternationalCABD351040glycerolSigma-AldrichG5516chloramphenicolSigma-AldrichC0378LBbrothFisherScientificBP1426-2DifcoAgarBDBiosciences214530glassbeadsFisherScientific10-310-1QFill3GenetixX3050BleachJavexEthanolSigma-AldrichE702315mLcentrifugetubesFisherScientific430052QPixcolonypickerGenetixQPix2pickinghead(E.coli)GenetixX4006
