MulticolorflowcytometryTechnicalResourceGuidehighperformanceimmunoassayshighperformanceimmunoassaysTableofContentsMulticolorFlowCytometry1QualityFirst.ValueAlways...................2UsingThisResourceGuide.................2ANoteRegardingData....................21.VioletLaser(405,407nm)3VioletLaserDyes........................3CompensationConsiderationsForVioletLaserDyes.4FixationConsiderationsForVioletLaserDyes......5ViabilityDetectionReagentsForTheVioletLaser...62.BlueLaser(488nm)7BlueLaserDyes.........................7CompensationConsiderationsForBlueLaserDyes..9FixationConsiderationsForBlueLaserDyes.......9ViabilityDetectionReagentsForTheBlueLaser...113.RedLaser(633nm)12RedLaserDyes........................12CompensationConsiderationsForRedLaserDyes.13FixationConsiderationsForRedLaserDyes......14ViabilityDetectionReagentsForTheRedLaser....15TenColorStainingExample16eBioscienceiscommittedtodevelopingandmanufacturinghigh-quality,innovativereagentsinanISOcertifiedfacility.Asaproviderofmorethan10,000products,weempowerourcustomersworldwidetoobtainexceptionalresultsbyusingreagentsthatofferanewstandardofexcellenceintheareasofinnovation,qualityandvalue.ForResearchUseOnly.Notforuseindiagnosticortherapeuticprocedures.eFluor®isaregisteredtrademarkofeBioscience,Inc.AlexaFluor®isaregisteredtrademarkof,andlicensedunderpatentsassignedtoMolecularProbes,Inc.(LifeTechnologies).Cy™,includingCy5,Cy5.5andCy7,isatrademarkofAmershamBiosciencesLtd.(GEHealthcare).1MulticolorFlowCytometryTechnicalResourceGuideFlowcytometryisanindispensabletechniquefordecipheringcomplexcellularprocessesandinteractionsinavarietyofsystemsthatmodelnormalanddiseasestates.Thedevelopmentofcytometersequippedwith3-5lasersandcapableofdetecting>15fluorescentparametershasmadeflowcytometryafundamentaltoolforthelifescientist.Realizingthepowerofmulticolorflowcytometryrequireshighperformancefluorophorespairedwithappropriatespecificitiestoensureconsistentandreliabledata.Astheindustryleaderinreagentofferingsformulticolo...
