JournalofVisualizedExperimentswww.jove.comCopyright©2010JournalofVisualizedExperimentsPage1of1MaterialsListfor:SDS-PAGE/ImmunoblotDetectionofAβMultimersinHumanCorticalTissueHomogenatesusingAntigen-EpitopeRetrievalRebeccaF.Rosen1,YasushiTomidokoro2,JorgeA.Ghiso3,LaryC.Walker1,41YerkesNationalPrimateResearchCenter,EmoryUniversity2DepartmentofNeurology,InstituteofClinicalMedicine,TsukubaUniversity3DepartmentofPathology,NewYorkUniversitySchoolofMedicine4DepartmentofNeurology,EmoryUniversityCorrespondenceto:RebeccaF.Rosenatrfrosen@emory.eduURL:http://www.jove.com/video/1916DOI:doi:10.3791/1916MaterialsNameCompanyCatalogNumberCommentsCompleteProteaseInhibitorCocktailTabletsSantaCruzBiotechnology,Inc.Sc-291301tabletin25mlbufferBCAProteinAssaykitThermoFisherScientific,Inc.23225XCellSureLockMini-CellandXCellIIBlotModuleKitCEMarkInvitrogenEI0002NovexTricineSDSSampleBuffer(2X)InvitrogenLC1676NuPAGESampleReducingAgent(10X)InvitrogenNP0004SeeBluePlus2Pre-StainedStandardInvitrogenLC5925Novex10-20%TricineGel1.0mm,10wellInvitrogenEC6625BOXNitrocellulosemembranes,0.2μmporesizeInvitrogenLC2000NovexTricineSDSRunningBuffer(10X)InvitrogenLC1675NovexTris-GlycineTransferBuffer(25X)InvitrogenLC3675SimplyBlueSafeStainInvitrogenLC6060WillnotinterferewithimmunostainingATXPonceauSRedstainingsolutionSigma-Aldrich09276WillnotinterferewithimmunostainingKapakheatsealableplasticsamplepouchesFisherScientific0181225AA6E10mousemonoclonalantibodytoAβ(1-16)CovanceSIG-39320Dilute1:1,000upto1:5,000forWBTween20Sigma-AldrichP2287ECLMouseIgG,HRP-LinkedWholeAβ(fromsheep)GEHealthcareNA931-1MLDiluteat1:10,000SuperSignalWestPicoChemiluminescentSubstrateThermoFisherScientific,Inc.34077KodakBiomaxMRFilmCarestreamHealth8701302
