JournalofVisualizedExperimentswww.jove.comCopyright©2012JournalofVisualizedExperimentsPage1of2MaterialsListfor:SingleOocyteBisulfiteMutagenesisMichelleM.Denomme1,2,3,LiyueZhang3,MellissaR.W.Mann1,2,31DepartmentofObstretrics&Gynaecology,SchulichSchoolofMedicineandDentistry,UniversityofWesternOntario2DepartmentofBiochemistry,SchulichSchoolofMedicineandDentistry,UniversityofWesternOntario3Children'sHealthResearchInstituteCorrespondenceto:MellissaR.W.Mannatmmann22@uwo.caURL:http://www.jove.com/video/4046DOI:doi:10.3791/4046MaterialsNameCompanyCatalogNumberCommentsOocyteCollectionHyaluronidaseSigmaH4272AcidicTyrodeSigmaT1788ProteinaseKSigmaP556810%IGEPALBioshopNON999.500LysisSolutionTrispH7.5BioshopTRS001.5LiClSigmaL9650EDTApH8.0SigmaE5134LiDSBioshopLDS701.10DTTInvitrogenP2325SDSLysisBufferTEpH7.5Bioshop(Tris)Sigma(EDTA)TRS001.5E513410%SDSBioshopSDS001.500BisulfiteConversionSodiumHydroxideSigmaS8045SodiumHydrogensulfite(SodiumBisulfite)Sigma243973HydroquinoneSigmaH9003LowMeltingPoint(LMP)AgaroseSigmaA9414MineralOilSigmaM8410M2MediumSigmaM7167GIBCODistilledwaterInvitrogen15230-196Autoclaveddoubledistilled(dd)waterPCRIllustraHotStartMixRTGGEHealthcare28-9006-54240ng/mlyeasttRNAInvitrogen15401-011InnerandouternestedprimersSigmaLigationPromegapGEM-TEasyVectorFisherScientificA1360TACloningCompetentE.colicellsZymoResearchCorp.T3009EquipmentJournalofVisualizedExperimentswww.jove.comCopyright©2012JournalofVisualizedExperimentsPage2of2DissectingMicroscope70°Cand90°CHeatBlocks37°Cand50°CWaterbaths(42°Cfortransformations)RockerPCRmachine
