JournalofVisualizedExperimentswww.jove.comCopyright©2009JournalofVisualizedExperimentsandStemgentPage1of1MaterialsListfor:CultureandMaintenanceofHumanEmbryonicStemCellsLiaKent11ResearchandDevelopment,StemgentCorrespondenceto:LiaKentatlia.kent@stemgent.comURL:http://www.jove.com/video/1427DOI:doi:10.3791/1427MaterialsNameCompanyCatalogNumberCommentsD-MEMInvitrogen11965-092ForMEFmediumFetalBovineSerum(FBS),CertifiedInvitrogen16000-044Heat-inactivatedForMEFmediumD-MEM/F-12Invitrogen11330-057Knockout™SerumReplacementInvitrogen10828-028Pre-screentoensuresupportofhEScellsbFGFStemgent03-0002Useat[4ng/mL]finalNon-essentialAminoAcidsInvitrogen11140-050L-glutamineInvitrogen25030-081200mM(100X)Useat[1X]finalPBSInvitrogen14190-250WithoutCa2+orMg2+CollagenaseIVInvitrogen17104-019Makeafreshsolutionat1mg/mLinD-MEM/F-12GelatinSigma-AldrichG1890TypeA,PorcineFetalBovineSerum(FBS),DefinedHycloneSH300.70.01Forfreezingmedium6-wellplatesNalgeNuncinternational140675Forgeneralculture4-wellplatesNalgeNuncinternational176740Forthawing5mLglasspipetsFisherScientific13-678-27EIndividuallywrapped15mLconicaltubesCorning430052PolypropylenePasteurpipettesFisherScientific13-678-20D9”glass
