Page1of2PRODUCTSPECIFICATIONSHEETHUMANFIBRONECTINFibronectinisabroadrangenaturalcelladhesionfactor.Itisa440-500kDadimericglycoproteinconsistingoftwosimilar220-250kDasubunitslinkedbytwodisulfidebonds.Itisfoundasadimerinplasmaandinmultimericformintheextracellularmatrixandoncellsurfaces.ItsprimaryfunctionisrelatedtocelladhesiontotheextracellularmatrixwhichoccursviatheArg-Gly-Asp-Ser(RGDS)sequenceoffibronectinwiththeappropriatetransmembraneintegrinreceptorofthecells.1Otherdomainsoffibronectinarealsoinvolvedwiththisadhesionprocessandmayinvolveinteractionwithcollagen,heparinandothercellsurfaceglycosaminoglycans.1Theconformationandorientationofadsorbedfibronectinisalsoimportantandhasaneffectoncellspreadingandstrengthofadhesionofendothelialcells.2Fibronectinadditiontoserumfreemediumpromotescelladhesion.3MoresignificanteffectsareobservedwithBHK,CHOandothercelllinesbycoatingofculturewarewithfibronectinat1-5ug/cm2.Detailsoffibronectinstructure,properties,distribution,cellularexpression,interactionwithotherproteins,matrixproperties,cellinteractionsandadhesionandeffectsondifferentiationcanbefoundintwoexcellentbooksbyHynesorMosher.4,5PRODUCT:FIBRONECTIN,HumanCATALOGNUMBER:354008LOTNUMBER:___________SOURCE:HumanplasmaNOTE:ThesourceplasmawastestedandfoundnonreactiveforhepatitisBsurfaceantigen(HBsAg)andnegativeforantibodytohumanimmunodeficiencyvirus(HIV).Nevertheless,thisproductshouldbehandledusingthesamesafetyprecautionsusedwhenhandlingpotentiallyinfectiousmaterial.QUANTITY&PHYSICALFORM:1milligrampervial,lyophilized.FORMULATION:100mMCAPS,0.15MNaCl,1mMcalciumchloride,pH11.0.RECONSTITUTION&USE:Equilibratevialtoroomtemperature.Resuspendinonemillilitersteriledistilledwater.Allow30minutesformaterialtogointosolution.DONOTAGITATEORSWIRL.Ifentireamountofmaterialisnottobeusedimmediately,transferintoappropriatealiquotsandstoreat-20oC.Itisrecommendedthatsolubilizedproductisusedwithintwoweeks.DONOTSTOREINFROST-FREEFREEZER.AVOIDMULTIPLEFREEZETHAWS.HumanFibronectinisgenerallyusedintheconcentr...
