JournalofVisualizedExperimentswww.jove.comCopyright©2013CreativeCommonsAttribution-NonCommercialLicensePage1of1MaterialsListfor:DetectionofProteinPalmitoylationinCulturedHippocampalNeuronsbyImmunoprecipitationandAcyl-BiotinExchange(ABE)G.StefanoBrigidi1,ShernazXBamji11DepartmentofCellularandPhysiologicalSciences,BrainResearchCentre,UniversityofBritishColumbiaCorrespondenceto:ShernazXBamjiatshernaz.bamji@ubc.caURL:http://www.jove.com/video/50031DOI:doi:10.3791/50031MaterialsNameCompanyCatalogNumberCommentsIGEPALCA-630SigmaI8896PMSFFluka93482ProteaseInhibitorCocktailRocheCompleteMini11836153001NEMSigmaE3876HAMsolutionSigma46780-4BCAProteinAssayKitThermoScientific23225EnsurecompatibilitywithchosendetergentProteinG/A-coatedsepharosebeadsGEHealthcare17-6002-35Biotin-BMCCThermoScientific21900Streptavidin-HRPThermoScientific21126Reconstituteat1mg/mlinwaterWesternBlotStrippingBufferThermoScientific21059
