JournalofVisualizedExperimentswww.jove.comCopyright©2012JournalofVisualizedExperimentsPage1of1MaterialsListfor:IsolationofRibosomeBoundNascentPolypeptidesinvitrotoIdentifyTranslationalPauseSitesAlongmRNASujataS.Jha1,AntonA.Komar11CenterforGeneRegulationinHealthandDisease,DepartmentofBiological,GeologicalandEnvironmentalSciences,ClevelandStateUniversityCorrespondenceto:AntonA.Komarata.komar@csuohio.eduURL:http://www.jove.com/video/4026DOI:doi:10.3791/4026MaterialsNameCompanyCatalogNumberCommentsMEGAscriptT7HighyieldTranscriptionKitAmbionAM1333RibonucleaseInhibitorInvitrogen15518012Trans[35S]-LabelMPBiomedicals0151006Ribonuclease-AInvitrogen12091RabbitReticulocyteLysateSystem,NucleaseTreatedPromegaL4960E.coliS30ExtractSystemforLinearTemplatesPromegaL1030CentrifugationBeckmanCoulterOptimaTLXUltracentrifugeStoragephosphorautoradiographyGEHealthcareTyphoon9410variablemodeimagerSoftwarefornascentpolypeptideanalysisGEHealthcareImageQuantTL,v2005
