JournalofVisualizedExperimentswww.jove.comCopyright©2012JournalofVisualizedExperimentsPage1of2MaterialsListfor:AssayingDNADamageinHippocampalNeuronsUsingtheCometAssaySomairaNowsheen1,FenXia2,EddyS.Yang1,31DepartmentofRadiationOncology,UniversityofAlabama-Birmingham2DepartmentofRadiationOncology,TheOhioStateUniversityMedicalSchool3DepartmentofCellBiology,andPharmacologyandToxicology,ComprehensiveCancerCenter,UniversityofAlabamaatBirminghamSchoolofMedicine,UniversityofAlabama-BirminghamCorrespondenceto:EddyS.Yangateyang@uab.eduURL:http://www.jove.com/video/50049DOI:doi:10.3791/50049MaterialsNameCompanyCatalogNumberComments1.5mltubesSantaCruzBiotechnology,IncSc-20027110XTBE(Trisbase,boricacid,EDTA)FisherScientificBP1333115mltubeFisherScientific0553851AgaroseSigmaA5093AluminumfoilFisherScientific01213101BeakerFisherScientificFB102300CentrifugeThermoScientific75004261PRCometAssaysoftwareCometAssayIVImageAnalysisSystemCylinderFisherScientific08555FEDTAGIBCO15575ElectrophoresischamberThermoScientific09528101EthanolFisherScientificA407P4FluorescentmicroscopeZeissAxioVisionAnyfluorescentmicroscopewithgreenfilterwillsufficeHemacytometerFisherScientific0267152LowmeltingpointagarosePromegaV2111MicrowaveSearsPhosphatebufferedsaline,calciumfree,magnesiumfreeHyCloneSH3025601PowersupplyBioRad1645050RefrigeratorSearsRulerStaplesSlideFisherScientific12550143SodiumchlorideSigmaS7653SodiumhydroxideSigmaS5881SodiumlaurylsarcosinateFisherScientificS529SybrgreenInvitrogenS7585TrayFisherScientific15242BTrisBaseSigmaT6066Triton-X100SigmaT8787TrypsinHyCloneSH3023601VortexFisherScientific02216108JournalofVisualizedExperimentswww.jove.comCopyright©2012JournalofVisualizedExperimentsPage2of2WaterbathFisherScientific154622Q
