JournalofVisualizedExperimentswww.jove.comCopyright©2011CreativeCommonsAttribution-NonCommercialLicensePage1of2MaterialsListfor:UsingWholeMountinsituHybridizationtoLinkMolecularandOrganismalBiologyNicoleL.Jacobs1,R.CraigAlbertson1,JasonR.Wiles1,21DepartmentofBiology,SyracuseUniversity2DepartmentofScienceTeaching,SyracuseUniversityCorrespondenceto:NicoleL.Jacobsatnljacobs@syr.edu,JasonR.Wilesatjwiles01@syr.eduURL:http://www.jove.com/video/2533DOI:doi:10.3791/2533MaterialsNameCompanyCatalogNumberComments5PrimeFastPlasmidMiniKit(100preps)FisherScientific2300000OneShotTOP10ChemicallyCompetentE.coliwithSOCMediumInvitrogenC404003LBAgarFisherScientificBP1425-500LBBrothFisherScientificBP1426-500AmpicillinSodiumSaltFisherScientificBP1760-5IsopropanolAcrosOrganics42383-0010PetriDish100x20mmnontreatedLaboratoryProductsSales43059114mlCultureTube,SnapTopFisherScientific1495911BRestrictionEnzymes&Buffers&10xBSANewEnglandBiolabsvariesDiethylPyrocarbonate(DEPC)25mlSigma-AldrichD5758-25mLSodiumAcetateTrihydrateUSP/FCC500gFisherScientifics608500Gal200proofEthylalcoholFisherScientific04-355-451Tris-Acetate-EDTA(TAE)50xSol1LFisherScientificbp13321AgaroseLowEEO100gFisherScientificBP160-100EthidiumBromide10mlSigma-Aldrich45-E1510SucroseGelLoadingDye40%SucroseFisherScientificBP655-11kbFullScaleDNALadderFisherScientificBP2582200DIGRNALabelingMixRocheGroup11277073910T3RNAPolymeraseRocheGroup1031163T7RNAPolymeraseRocheGroup10881767001SP6RNAPolymeraseRocheGroup810274ProtectorRnaseInhibitorRocheGroup3335399001DnaseI,RnaseFree10,000unitsRocheGroup4716728001EDTAmolecularbiologyreagentSigma-Aldriche5134-500GLithiumChloride100gFisherScientificL121100SodiumCarbonate1kgFisherScientificBP357-1SodiumBicarbonate,500gFisherScientificBP328-500JournalofVisualizedExperimentswww.jove.comCopyright©2011CreativeCommonsAttribution-NonCommercialLicensePage2of2AceticAcidglacialACS500mlFisherScientifica38500ParaformaldehydeR500gFisherScientifico4042500PBSPhosphateBufferSaline10XFisherScientificbp3991Tween2050...
