JournalofVisualizedExperimentswww.jove.comCopyright©2011CreativeCommonsAttribution-NonCommercialLicensePage1of1MaterialsListfor:IsolationofTranslatingRibosomesContainingPeptidyl-tRNAsforFunctionalandStructuralAnalysesNitinShirole1,SreeramBalasubramanian1,CharlesYanofsky2,LuisCruz-Vera11DepartmentofBiologicalSciences,UniversityofAlabamaHuntsville2DepartmentofBiology,StanfordUniversityCorrespondenceto:LuisCruz-Veraatlrc0002@uah.eduURL:http://www.jove.com/video/2498DOI:doi:10.3791/2498MaterialsNameCompanyCatalogNumberCommentsTrisbaseSigma-Aldrich252859MagnesiumacetateFluka63049PotassiumglutamateSigma-AldrichG1501AmmoniumacetateFluka09688PMSFSigma-AldrichP7626ProteinAsepharose4BslurrybeadsSigma-AldrichP9424LeupeptininhibitorSigma-AldrichL9783Taq-DNApolymeraseStratagene,AgilentTechnologies201223T7MaxiprepkitPromegaCorp.P1300SMBPromegaCorp.Z5482Biotin-16-UTPRocheGroup1388908ATPSigma-AldrichA7699GTPSigma-AldrichG8877PEPSigma-AldrichP7002PKSigma-AldrichP7768Polyethylenglycol8000Fluka81268FolinicacidFluka47612SpermidineFluka85558tRNAfromE.coliSigma-AldrichR1753DEPCSigma-AldrichD5758TricineSigma-AldrichT5816L-aminoacidsSigma-AldrichLAA21DTTFluka43815magneticseparationstandsPromegaCorp.Z5342
